KIF7 Protein (AA 1-1348) (His tag)

Catalog No. ABIN7562070

Product Details

Target: KIF7 (Kinesin Family Member 7 (KIF7))

Protein Type: Recombinant

Protein Characteristics: AA 1-1348

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This KIF7 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Kif7 Protein expressed in mammalian cells.

Sequence: MGLEAQRLPG AEEAPVRVAL RVRPLLPKEL LHGHQSCLRV EPERGRITLG RDRHFGFHVV LGEDTGQEAV YQACVQPLLE AFFEGFNATV FAYGQTGSGK TYTMGEASVA SLHEDEQGII PRAMAEAFKL IDENDLLDCL VHVSYLELYK EEFRDLLEVG TASRDIQLRE DDRGNVVLCG VKEVDVEGLD EVLSLLEMGN AARHTGATHF NRLSSRSHTV FTVTLEQRGR TPSRLPRPAA GHLLVSKFHF VDLAGSERVL KTGSTGERLK ESIQINSTLL ALGNVISALG DPQRRGSHIP YRDSKITRIL KDSLGGNAKT VMIACVSPSS SDFDETLNTL NYASRAQNIR NRATVNWHPE AERVPEEQAA GARGPPRHRS ETRIIHRGRR VPCPAVGSAA VAAGLGAECA RCRARTSAAY SLLRELQAEP GLPGAAARKV RDWLCAVEGE RSTLSSASGP DSGIESAPAE DQAAQGTSGR KGDEGTQQLL TLQSQVARLE EENRDFLAAL EDAMEQYKLQ SDRLREQQEE MVELRLRLEL AQPGWGAPGL LQGLPPGSFV PRPHTAPLGG AHTHMLGMMP STCLPGEEVS SEQQVVSGKE VKAEVLAQAD KLRSASSTTS EEEGEEEEEE EEEEEEPPRR TLYLRRNGIS NWSQRAGLSP GSPPDRKGPE VCPEEPAAAI PAPQAVGSGK VPVQTRQAPA AMASEWRLAQ AQQKIRELAI NIRMKEELIG ELVRTGKAAQ ALNRQHSQRI RELEQEAERV RAELCEGQRQ LRELEGREPQ DASERSRLQE FRKRVAAAQS QVQVLKEKKQ ATERLVSLSA QSETRLQELE RNVQLMRRQQ GQLQRRLREE TEQKRRLETE MNKRQHRVKE LELKHEQQQK ILKIKTEEIA AFQRKRRSGS NGSVVSLEQQ QKIEEQKKWL DQEMEKVLQQ RRALEELGEE LRKREVILAK KEALMQEKTG LESKRLRSSQ ALNEDIVRVS SRLEHLEKEL SEKSGQLRQG SAQNQQQIRG EIDTLRQEKD SLLKQRLEID SKLRQGSLLS PEEERTLFQL DEAIEALDAA IEYKNEAITC RQRVLRASAS LLSQCEMNLM AKLSYLSSSE TRALLCKYFD KVVTLREEQH QQQIAFSELE MQLEEQQRLV YWLEVALERQ RLEMDRQLTL QQKEHEQNVQ LLLQQGRDHL GEGLADSKRQ YEARIHALEK ELGRHMWINQ ELKQKLSAGS TAGQSQGCER RSLCLENRQC LGNEDGLHPA APEPLWQSSL LEGVSRVWDE SRDLVHAPLP LTWKRSSLCS EQGSSEESRV RETTEPPVGR VLPMGEVGLS WNFGPLPKPR WEPRRTSPGM IDVRKNPL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: KIF7 (Kinesin Family Member 7 (KIF7))

Alternative Name: Kif7 (KIF7 Products)

Background: Kinesin-like protein KIF7,FUNCTION: Essential for hedgehog signaling regulation: acts both as a negative and a positive regulator of sonic hedgehog (Shh) and Indian hedgehog (Ihh) pathways, acting downstream of SMO, through both SUFU-dependent and -independent mechanisms. Involved in the regulation of microtubular dynamics. Required for proper organization of the ciliary tip and control of ciliary localization of SUFU-GLI2 complexes. Required for localization of GLI3 to cilia in response to Shh. Negatively regulates Shh signaling by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates Shh signaling by preventing the processing of the transcription factor GLI3 into its repressor form. In keratinocytes, promotes the dissociation of SUFU-GLI2 complexes, GLI2 nuclear translocation and Shh signaling activation. Involved in the regulation of epidermal differentiation and chondrocyte development. {ECO:0000269|PubMed:19549984, ECO:0000269|PubMed:19592253, ECO:0000269|PubMed:19666503, ECO:0000269|PubMed:21795282, ECO:0000269|PubMed:23034632, ECO:0000269|PubMed:24952464}.

Molecular Weight: 151.6 kDa

UniProt: B7ZNG0

AlphaFold: B7ZNG0

Pathways: Hedgehog Signaling