PTCHD4 Protein (AA 1-904) (His tag)

Catalog No. ABIN7562078

This Recombinant PTCHD4 protein is expressed in HEK-293 Cells.

Quick Overview for PTCHD4 Protein (AA 1-904) (His tag) (ABIN7562078)

Target: PTCHD4 (Patched Domain Containing 4 (PTCHD4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-904

Purification tag / Conjugate: This PTCHD4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ptchd4 Protein expressed in mammalian cells.

Sequence: MCFLRRPGAP ASWIWWRMLR QVLRRGLQSF CHRLGLCVSR HPVFFLTVPA VLTITFGLSA LNRFQTEGDL ERLVAPSHSL AKIERSLASS LFPLDQSKSQ LYSDLHTPGR YGRVILLSSP GDNILLQAEG ILQTHRAVME MKVNHKGYNY TFSHLCVLRN QDKKCVLDDI ISVLEDLRQA AVSNKTTARV QVRYPNTKLK DGRNSFIGHQ LGGVVEVPNS KDQRVKSARA IQITYYLQTY GSATQDLIGE KWENEFCKLM RKLQEEHQDL QLYSLASFSL WRDFHKTSIL TRSKVLVSLV LILTTATLSS SMKDCLRSKP FLGLLGVLTV CISIATAAGI FFITDGKYNS TLLGIPFFAM GHGTKGVFEL LSGWRRTKEN LPFKDRVADA YSDVMVTYTM TSSLYFITFG MGASPFTNIE AVKIFCQNMC VSILLNYFYI FSFFGSCLVF AGQLEQNRYH SIFCCKIPSA EYLDRKPVWF QTVMSDGHQQ TSHHETNPYQ HHFIQHFLRE HYNEWITNIY VKPFVVILYL IYASFSFMGC LQISDGASII NLLASDSPSV SYAMVQQKYF SNYSPVIGFY VYEPLEYWNS SVQEDLQRLC SGFTAVSWVE QYYQFLKTSN ISANNKTDFI SVLQSSFLKK PEFQHFRNDI IFSRAGDENN IIASRLYLVA RTSRDKQKEV IEVLDKLRPL SLSKSIRFIV FNPSFVFTDH YSLSVTVPVL IAGFGVLLVL ILTFFLVIHP LGNFWLILSV TSIELGVLGL MTLWNVDMDC ISILCLIYTL NFAIDHCAPL LYTFVLATEH TRTQCIKSSL QEHGTAILQN ITSFLIGLLP LLFVPSNLTF TLFKCLLLTG GCTLLHCFVI LPVFLTFFPP SKKHHKKKKR AKRKEREEIE CIEIQENPDH VTTV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PTCHD4 (Patched Domain Containing 4 (PTCHD4))

Alternative Name: Ptchd4

Background: Patched domain-containing protein 4,FUNCTION: Could act as a repressor of canonical hedgehog signaling by antagonizing the effects of SMO, as suggested by down-regulation of hedgehog target genes, including GLI1, PTCH1, and PTCH2 in PTCHD4-expressing cells. {ECO:0000250|UniProtKB:Q6ZW05}.

Molecular Weight: 103.1 kDa

UniProt: B9EKX1

AlphaFold: B9EKX1