RNF217 Protein (AA 1-515) (His tag)
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- Target See all RNF217 Proteins
- RNF217 (Ring Finger Protein 217 (RNF217))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-515
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RNF217 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Rnf217 Protein expressed in mammalian cells.
- Sequence
- MGEEQSTVSG SGGARASGGG SAGQPESPRP RGDRVRTAGP RAAASSSRPN GGGGGRDPGC VDASVQEPAS NRAPAGQPAR LPLSGPLDPQ SLELQLEREA EGAGPREAPP GQQPPDGLLL DVLAQRHPPP AKPQVLCSVY CVESDLPEAP SAESPSPSES PPQAPLGPIP ASPPPSFPSS PLSLPADPLS PDGGSIELEF YLAPEPFSVP GLLGAPPYSG LGGVGDPYAP LMVLMCRVCL EDKPIKPLPC CKKAVCEECL KIYLSSQVQL GQVEIKCPVT ECFEFLEETT VVYNLTHEDS IKYKYFLELG RIDSSTKPCP QCKHFTTFKK KGHIPTPSRS ESRYKIQCPT CQLIWCFKCH SPWHEGVNCK EYKKGDKLLR HWASEIEHGQ RNAQKCPKCK IHIQRTEGCD HMTCSQCNTN FCYRCGERYR QLRFFGDHTS NLSIFGCKYR YLPERPHLRR LVRGSVCAGK LFIAPLILVL GLALGAIAVV IGLFVFPIYC LCKKQRKRSR TGMHW Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Ring Finger Protein 217 (RNF217) (AA 1-542) protein (His tag)
custom-made RNF217 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 217 (RNF217) (AA 2-100) protein (GST tag)RNF217 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- RNF217 (Ring Finger Protein 217 (RNF217))
- Alternative Name
- Rnf217 (RNF217 Products)
- Background
- E3 ubiquitin-protein ligase RNF217 (EC 2.3.2.31) (IBR domain-containing protein 1) (RING finger protein 217),FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis. {ECO:0000269|PubMed:33895792}.
- Molecular Weight
- 56.0 kDa
- UniProt
- D3YYI7
- AlphaFold
- D3YYI7
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