C6orf192 Protein (AA 1-459) (His tag)

Catalog No. ABIN7562108

Product Details

Target: C6orf192 (Chromosome 6 Open Reading Frame 192 (C6orf192))

Protein Type: Recombinant

Protein Characteristics: AA 1-459

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This C6orf192 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Slc18b1 Protein expressed in mammalian cells.

Sequence: MDEAGSPAPA GTGGGDDPGG STRETSRRLS REQIFVLVSA ASMNLGCMMT YSILGPFFPK EAEKKGASNT MIGMIFGCYA LFELLASLVF GKYLVHIGAK FMFIAGMFVS GGVTILFGVL DQLPEGPIFI AMCFLVRIVD AIGFGAAITA SSSILAKAFP NNVATVMGSL EVFSGLGLVA GPPLGGLLYQ SFGYEVPFIF LGCIVLLMIP LNLYILPSYA QESDPGKQSF WKLVTLPKMG LLAFVIISLS SCFGFLDPTL SLFVMEKFSL STGYVGLVFL GLSLSYAISS PLFGLLSDKM PTLRKWLLVF GNLITAGCYM LLGPVPLLHI KSQLWLLVLV LVVNGISAGM SIIPTFPEML SCAYANGFED SISTLGLVSG LFGAMWSVGA FMGPILGGFL CEKIGFEWAA AMQGLWTLLS GVSMALFYLW EDSTARRRSK AQNSLGTEEE RAALLPNDT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: C6orf192 (Chromosome 6 Open Reading Frame 192 (C6orf192))

Alternative Name: Slc18b1 (C6orf192 Products)

Background: MFS-type transporter SLC18B1 (Solute carrier family 18 member B1) (Vesicular polyamine transporter) (VPAT),FUNCTION: Proton-coupled polyamine antiporter involved in the translocation of polyamines from cytosol into secretory vesicles prior to their release via exocytosis. Uses the electrochemical proton gradient generated by a V-type proton-pumping ATPase to couple the efflux of protons with the uptake of a polyamine molecule (By similarity). Facilitates vesicular storage of spermine and spermidine in astrocytes with an impact on glutamatergic neuronal transmission and memory formation (PubMed:31800589) (By similarity). Upon antigen stimulation, regulates polyamine accumulation and release in mast cell secretory granules, which in turn potentiates mast cell degranulation and histamine secretion (PubMed:28082679). {ECO:0000250|UniProtKB:D4A9K4, ECO:0000250|UniProtKB:Q6NT16, ECO:0000269|PubMed:28082679, ECO:0000269|PubMed:31800589}.

Molecular Weight: 49.0 kDa

UniProt: D3Z5L6

AlphaFold: D3Z5L6