SP100 Protein (AA 1-482) (His tag)

Catalog No. ABIN7562382

Product Details

Target: SP100 (SP100 Nuclear Antigen (SP100))

Protein Type: Recombinant

Protein Characteristics: AA 1-482

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This SP100 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Sp100 Protein expressed in mammalian cells.

Sequence: MEGSDGSPRM STEQENTEMH LIECMLKHFK TQKVAISNAI RSTFPFLESL RDHEFITGKM YEDLLDSCRS LVPVDKVIYR ALEELEKKFD MTVLCELFNE VNMEKYPDLN LIRRSFGCVF PNELCFQGID GGNPNSQLSL EQGPGASYSQ GSPNGSSLDL SASEGWRSND RRNSNLMQAN QTENHQLAES PGHLDSCELQ VQLNNRDATP ESCSLLPQNE ERAVQLNYEL QINPCFVQLV DVKKENSSFS LAGNQQTRAR TNQNEDSEII ELSSGDSDNG ENFSEATTTV PSQPAPAYSR KPPTLRRDRG GDTSDTESSI IIRRRKRTGR KKRERLGSYL IRNIKIPMKP SWKTAFLARS ANPSSQRRRK RGPRIPREEN ADFGGAELPV VCGNAQGFLD KEKFKQGIYV RSIRGKTGRL FTPMDFEIEG NCEKAKNWRQ SIRCKGWTLR ELIQKGVLQD PPRKKKETPR NPRQTRRQVN AL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SP100 (SP100 Nuclear Antigen (SP100))

Alternative Name: Sp100 (SP100 Products)

Background: Nuclear autoantigen Sp-100 (Nuclear dot-associated Sp100 protein) (Speckled 100 kDa),FUNCTION: Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity). {ECO:0000250|UniProtKB:P23497}.

Molecular Weight: 54.7 kDa

UniProt: O35892

AlphaFold: O35892

Pathways: Retinoic Acid Receptor Signaling Pathway