PTRF Protein (AA 1-392) (His tag)

Catalog No. ABIN7562402

Product Details

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Protein Type: Recombinant

Protein Characteristics: AA 1-392

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PTRF protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Cavin1 Protein expressed in mammalian cells.

Sequence: MEDVTLHIVE RPYSGFPDAS SEGPEPTQGE ARATEEPSGT GSDELIKSDQ VNGVLVLSLL DKIIGAVDQI QLTQAQLEER QAEMEGAVQS IQGELSKLGK AHATTSNTVS KLLEKVRKVS VNVKTVRGSL ERQAGQIKKL EVNEAELLRR RNFKVMIYQD EVKLPAKLSV SKSLKESEAL PEKEGDELGE GERPEDDTAA IELSSDEAVE VEEVIEESRA ERIKRSGLRR VDDFKKAFSK EKMEKTKVRT RENLEKTRLK TKENLEKTRH TLEKRMNKLG TRLVPVERRE KLKTSRDKLR KSFTPDHVVY ARSKTAVYKV PPFTFHVKKI REGEVEVLKA TEMVEVGPED DEVGAERGEA TDLLRGSSPD VHTLLEITEE SDAVLVDKSD SD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Alternative Name: Cavin1 (PTRF Products)

Background: Caveolae-associated protein 1 (Cav-p60) (Cavin-1) (Polymerase I and transcript release factor),FUNCTION: Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues (PubMed:18191225, PubMed:18840361, PubMed:18056712, PubMed:30188967). Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1) (PubMed:19546242). Essential for normal oligomerization of CAV1 (PubMed:23652019). Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop (PubMed:27528195). Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (PubMed:9582279, PubMed:11139612, PubMed:10589839). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (PubMed:30188967). {ECO:0000269|PubMed:10589839, ECO:0000269|PubMed:11139612, ECO:0000269|PubMed:18056712, ECO:0000269|PubMed:18191225, ECO:0000269|PubMed:18840361, ECO:0000269|PubMed:19546242, ECO:0000269|PubMed:23652019, ECO:0000269|PubMed:27528195, ECO:0000269|PubMed:30188967, ECO:0000269|PubMed:9582279}.

Molecular Weight: 44.0 kDa

UniProt: O54724

AlphaFold: O54724