ATP10A Protein (AA 1-1508) (His tag)

Catalog No. ABIN7562424

Product Details

Target: ATP10A (ATPase, Class V, Type 10A (ATP10A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1508

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ATP10A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Atp10a Protein expressed in mammalian cells.

Sequence: MERELPAAEE SASSGWRRPR RRRWEGRTRT VRSNLLPPLG TEDSTIGAPK GERLLMRGCI QHLADNRLKT TKYTLLSFLP KNLFEQFHRL ANVYFVFIAL LNFVPAVNAF QPGLALAPVL FILAVTAIKD LWEDYSRHRS DHEINHLGCL VFSREEKKYV NRYWKEIRVG DFVRLCCNEI IPADILLLSS SDPDGLCHIE TANLDGETNL KRRQVVRGFS ELVSEFNPLT FTSVIECEKP NNDLSRFRGY IMHSNGEKAG LHKENLLLRG CTIRNTEAVA GIVIYAGHET KALLNNSGPR YKRSQLERQM NCDVLWCVLL LVCISLFSAV GHGLWVRRYQ EKKALFDVPE SDGSSLSPAT AAVYSFFTMI IVLQVLIPIS LYVSIEIVKV CQVYFINQDI ELYDEETDSQ LQCRALNITE DLGQIKYIFS DKTGTLTENK MVFRRCTVSG IEYSHDANAQ RLARYQEADS EEEEVVSKVG TISHRGSTGS HQSIWMTHKT QSIKSHRRTG SRAEAKRASM LSKHTAFSSP MEKDITPDPK LLEKVSECDR FLAIARHQEH PLAHLSPELS DVFDFFIALT ICNTVVVTSP DQPRQKVRVR FELKSPVKTI EDFLRRFTPS RLASGCSSIG NLSTSKSSHK SGSAFLPSLS QDSMLLGLEE KLGQTAPSIA SNGYASQAGQ EESWASECTT DQKCPGEQRE QQEGELRYEA ESPDEAALVY AARAYNCALV DRLHDQVSVE LPHLGRLTFE LLHTLGFDSI RKRMSVVIRH PLTDEINVYT KGADSVVMDL LLPCSSDDAR GRHQKKIRSK TQNYLNLYAV EGLRTLCIAK RVLSKEEYAC WLQSHIEAEA SVESREELLF QSAVRLETNL HLLGATGIED RLQEGVPETI AKLRQAGLQI WVLTGDKQET AINIAYACKL LDHGEEVITL NADSQEACAA LLDQCLSYVQ SRNPRSTLQN SESNLSVGFS FNPVSTSTDA SPSPSLVIDG RSLAYALEKS LEDKFLFLAK QCRSVLCCRS TPLQKSMVVK LVRSKLKAMT LAIGDGANDV SMIQVADVGV GISGQEGMQA VMASDFAVPR FRYLERLLIV HGHWCYSRLA NMVLYFFYKN TMFVGLLFWF QFYCGFSASA MIDQWYLIFF NLLFSSLPQL VTGVLDKDVP ADMLLREPQL YKSGQNMEEY RPRAFWLNMV DAAFQSLVCF FIPYLAYYDS DVDVFTWGTP VTAIALFTFL LHLGIETKTW TWLNWLACGF STFLFFSVAL IYNTSCATCY PPSNPYWTMQ TLLGDPLFYL TCLIAPIAAL LPRLFFKALQ GSLFPTQLQL GRQLAKKPLN KFSDPKETFA QGQPPGHSET ELSERKTMGP FETLPRDCAS QASQFTQQLT CSPEASGEPS AVDTNMPLRE NTLLEGLGSQ ASGSSMPRGA ISEVCPGDSK RQSTSASQTA RLSSLFHLPS FGSLNWISSL SLASGLGSVL QLSGSSLQMD KQDGEFLSNP PQPEQDLHSF QGQVTGYL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ATP10A (ATPase, Class V, Type 10A (ATP10A))

Alternative Name: Atp10a (ATP10A Products)

Background: Phospholipid-transporting ATPase VA (EC 7.6.2.1) (ATPase class V type 10A) (P-locus fat-associated ATPase) (P4-ATPase flippase complex alpha subunit ATP10A),FUNCTION: Catalytic component of P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of phosphatidylcholine (PC) from the outer to the inner leaflet of the plasma membrane. Initiates inward plasma membrane bending and recruitment of Bin/amphiphysin/Rvs (BAR) domain-containing proteins involved in membrane tubulation and cell trafficking. Facilitates ITGB1/beta1 integrin endocytosis, delaying cell adhesion and cell spreading on extracellular matrix. Has low flippase activity toward glucosylceramide (GlcCer). {ECO:0000250|UniProtKB:O60312}.

Molecular Weight: 168.8 kDa

UniProt: O54827

AlphaFold: O54827