Ribonuclease H1 Protein (RNASEH1) (AA 1-285) (His tag)
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- Target See all Ribonuclease H1 (RNASEH1) Proteins
- Ribonuclease H1 (RNASEH1)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-285
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Ribonuclease H1 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Rnaseh1 Protein expressed in mammalian cells.
- Sequence
- MRWLLPLSRT VTLAVVRLRR GICGLGMFYA VRRGRRTGVF LSWSECKAQV DRFPAARFKK FATEDEAWAF VRSSSSPDGS KGQESAHEQK SQAKTSKRPR EPLGEGEELP EPGPKHTRQD TEPAAVVSKD TFSYMGESVI VYTDGCCSSN GRKRARAGIG VYWGPGHPLN VGIRLPGRQT NQRAEIHAAC KAIMQAKAQN ISKLVLYTDS MFTINGITNW VQGWKKNGWR TSTGKDVINK EDFMELDELT QGMDIQWMHI PGHSGFVGNE EADRLAREGA KQSED Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product RNASEH1 Protein
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Ribonuclease H1 (RNASEH1) (AA 1-286) protein (His tag)
custom-made RNASEH1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ribonuclease H1 (RNASEH1) (AA 189-286) protein (GST tag)RNASEH1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ribonuclease H1 (RNASEH1) protein (Myc-DYKDDDDK Tag)RNASEH1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Ribonuclease H1 (RNASEH1)
- Alternative Name
- Rnaseh1 (RNASEH1 Products)
- Background
- Ribonuclease H1 (RNase H1) (EC 3.1.26.4),FUNCTION: Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II. {ECO:0000250|UniProtKB:O60930}.
- Molecular Weight
- 31.8 kDa
- UniProt
- O70338
- AlphaFold
- O70338
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