GNPDA1 Protein (AA 1-289) (His tag)

Catalog No. ABIN7562696

Product Details

Target: GNPDA1 (Glucosamine-6-Phosphate Deaminase 1 (GNPDA1))

Protein Type: Recombinant

Protein Characteristics: AA 1-289

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This GNPDA1 protein is labelled with His tag.

Purpose: Made-to-order recombinant Gnpda1 Protein expressed in mammalian cells.

Sequence: MKLIILEHYS QASEWAAKYI RNRIIQFNPG PDKYFTLGLP TGSTPLGCYQ KLIEYYKNGD LSFQYVKTFN MDEYVGLPRD HPESYHSFMW NNFFKHIDIH PENTHILDGN AADLQAECDA FEEKIQAAGG IELFVGGIGP DGHIAFNEPG SSLVSRTRVK TLAMDTILAN ARFFDGDLAK VPTMALTVGV GTVMDAKEVM ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTVFVCD EDATLELKVK TVKYFKGLML VHNKLVDPLY SIKEKEIQKS QSAKKPYSD Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GNPDA1 (Glucosamine-6-Phosphate Deaminase 1 (GNPDA1))

Alternative Name: Gnpda1 (GNPDA1 Products)

Background: Glucosamine-6-phosphate isomerase 1 (EC 3.5.99.6) (Glucosamine-6-phosphate deaminase 1) (GNPDA 1) (GlcN6P deaminase 1) (Oscillin),FUNCTION: Catalyzes the reversible conversion of alpha-D-glucosamine 6-phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway. Deamination is coupled to aldo-keto isomerization mediating the metabolic flux from UDP-GlcNAc toward Fru-6P. At high ammonium level can drive amination and isomerization of Fru-6P toward hexosamines and UDP-GlcNAc synthesis (By similarity). Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and their effects on hyaluronan synthesis that occur during tissue remodeling (By similarity). Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo (By similarity). {ECO:0000250|UniProtKB:P46926, ECO:0000250|UniProtKB:Q64422}.

Molecular Weight: 32.5 kDa

UniProt: O88958

AlphaFold: O88958