ABCD4 Protein (AA 1-606) (His tag)

Catalog No. ABIN7562703

Product Details

Target: ABCD4 (ATP-Binding Cassette, Sub-Family D (Ald), Member 4 (ABCD4))

Protein Type: Recombinant

Protein Characteristics: AA 1-606

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ABCD4 protein is labelled with His tag.

Purpose: Made-to-order recombinant Abcd4 Protein expressed in mammalian cells.

Sequence: MAVPGPTARA GARPRLDLQL VQRFVRIQKV FFPSWSSQNV LMFMTLLCVT LLEQLVIYQV GLIPSQYYGV LGNKDLDGFK ALTLLAVTLI VLNSTLKSFD QFTCNLLYVS WRKDLTEHLH HLYFRARVYY TLNVLRDDID NPDQRISQDV ERFCRQLSSV TSKLIISPFT LTYYTYQCFQ STGWLGPVSI FGYFIVGTMV NKTLMGPIVT KLVQQEKLEG DFRFKHMQIR VNAEPAAFYR AGLVEHMRTD RRLQRLLQTQ RELMSRELWL YIGINTFDYL GSILSYVVIA IPIFSGVYGD LSPTELSTLV SKNAFVCIYL ISCFTQLIDL STTLSDVAGY THRIGELQEA LLDMSRKSQD CEALGESEWD LDKTPGCPTT EPSDTAFLLD RVSILAPSSD KPLIKDLSLK ICEGQSLLIT GNTGTGKTSL LRVLGGLWEG MKGSVQMLAD FGPHGVLFLP QKPFFTDGTL REQVIYPLKE IYPDSGSADD ERIVRFLELA GLSSLVARTG GLDQQVDWNW YDVLSPGEMQ RLSFARLFYL QPKYAVLDEA TSALTEEAES ELYRIGQQLG MTFISVGHRP SLEKFHSWVL RLHGGGSWEL TRIKLE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ABCD4 (ATP-Binding Cassette, Sub-Family D (Ald), Member 4 (ABCD4))

Alternative Name: Abcd4 (ABCD4 Products)

Background: Lysosomal cobalamin transporter ABCD4 (EC 7.6.2.8) (ATP-binding cassette sub-family D member 4) (PMP70-related protein) (P70R) (Peroxisomal membrane protein 1-like) (PXMP1-L) (Peroxisomal membrane protein 69) (PMP69),FUNCTION: Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner. Targeted by LMBRD1 lysosomal chaperone from the endoplasmic reticulum to the lysosomal membrane. Then forms a complex with lysosomal chaperone LMBRD1 and cytosolic MMACHC to transport cobalamin across the lysosomal membrane. {ECO:0000250|UniProtKB:O14678}.

Molecular Weight: 68.6 kDa

UniProt: O89016

AlphaFold: O89016