FOSB Protein (AA 1-338) (His tag)

Catalog No. ABIN7562858

This Recombinant FOSB protein is expressed in HEK-293 Cells.

Quick Overview for FOSB Protein (AA 1-338) (His tag) (ABIN7562858)

Target: FOSB (FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-338

Purification tag / Conjugate: This FOSB protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Fosb Protein expressed in mammalian cells.

Sequence: MFQAFPGDYD SGSRCSSSPS AESQYLSSVD SFGSPPTAAA SQECAGLGEM PGSFVPTVTA ITTSQDLQWL VQPTLISSMA QSQGQPLASQ PPAVDPYDMP GTSYSTPGLS AYSTGGASGS GGPSTSTTTS GPVSARPARA RPRRPREETL TPEEEEKRRV RRERNKLAAA KCRNRRRELT DRLQAETDQL EEEKAELESE IAELQKEKER LEFVLVAHKP GCKIPYEEGP GPGPLAEVRD LPGSTSAKED GFGWLLPPPP PPPLPFQSSR DAPPNLTASL FTHSEVQVLG DPFPVVSPSY TSSFVLTCPE VSAFAGAQRT SGSEQPSDPL NSPSLLAL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FOSB (FBJ Murine Osteosarcoma Viral Oncogene Homolog B (FOSB))

Alternative Name: Fosb

Background: Protein FosB (FBJ osteosarcoma oncogene B) (Transcription factor AP-1 subunit FosB),FUNCTION: Heterodimerizes with proteins of the JUN family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to gene promoters containing an AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing their transcriptional activity (PubMed:2498083, PubMed:1900040). As part of the AP-1 complex, facilitates enhancer selection together with cell-type-specific transcription factors by collaboratively binding to nucleosomal enhancers and recruiting the SWI/SNF (BAF) chromatin remodeling complex to establish accessible chromatin (PubMed:29272704). Together with JUN, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (By similarity). Exhibits transactivation activity in vitro (PubMed:17241283). Involved in the display of nurturing behavior towards newborns (PubMed:8706134). May play a role in neurogenesis in the hippocampus and in learning and memory-related tasks by regulating the expression of various genes involved in neurogenesis, depression and epilepsy (PubMed:23303048, PubMed:30902680). Implicated in behavioral responses related to morphine reward and spatial memory (PubMed:9294222, PubMed:18407360). {ECO:0000250|UniProtKB:P53539, ECO:0000269|PubMed:17241283, ECO:0000269|PubMed:18407360, ECO:0000269|PubMed:1900040, ECO:0000269|PubMed:23303048, ECO:0000269|PubMed:2498083, ECO:0000269|PubMed:29272704, ECO:0000269|PubMed:30902680, ECO:0000269|PubMed:8706134, ECO:0000269|PubMed:9294222}., FUNCTION: [Isoform 2]: Exhibits lower transactivation activity than isoform 1 in vitro (PubMed:17241283). The heterodimer with JUN does not display any transcriptional activity, and may thereby act as an transcriptional inhibitor (PubMed:1900040). May be involved in the regulation of neurogenesis in the hippocampus (PubMed:23303048). May play a role in synaptic modifications in nucleus accumbens medium spiny neurons and thereby play a role in adaptive and pathological reward-dependent learning, including maladaptive responses involved in drug addiction (PubMed:23319622). Seems to be more stably expressed with a half-life of ~9.5 hours in cell culture as compared to 1.5 hours half-life of isoform 1 (PubMed:18407360). {ECO:0000269|PubMed:17241283, ECO:0000269|PubMed:18407360, ECO:0000269|PubMed:1900040, ECO:0000269|PubMed:23303048, ECO:0000269|PubMed:23319622}.

Molecular Weight: 36.0 kDa

UniProt: P13346

AlphaFold: P13346