POU3F3 Protein (AA 1-497) (His tag)
custom-made
POU3F3
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all POU3F3 Proteins
- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-497
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POU3F3 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Pou3f3 Protein expressed in mammalian cells.
- Sequence
- MATAASNPYL PGNSLLTAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM QPGSAAVTSG AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL PHAAAAAAAA AAAAVEASSP WSGSAVGMAG SPQQPPQPPP PPPQGPDVKG GAGREDLHAG TALHHRGPPH LGPPPPPPHQ GHPGGWGAAA AAAAAAAAAA AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG GGGGGAGGGA QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN VFSQTTICRF EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ GRKRKKRTSI EVSVKGALES HFLKCPKPSA QEITNLADSL QLEKEVVRVW FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT VSADTPPPHH GLQTSVQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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POU Class 3 Homeobox 3 (POU3F3) (AA 1-500) protein (His tag)
custom-made POU3F3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
- Alternative Name
- Pou3f3 (POU3F3 Products)
- Background
- POU domain, class 3, transcription factor 3 (Brain-specific homeobox/POU domain protein 1) (Brain-1) (Brn-1) (Octamer-binding protein 8) (Oct-8) (Octamer-binding transcription factor 8) (OTF-8),FUNCTION: Transcription factor that acts synergistically with SOX11 and SOX4 (By similarity). Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction, the enhancer element contains the octamer motif (5'-ATTTGCAT-3'). {ECO:0000250|UniProtKB:Q63262, ECO:0000269|PubMed:10473120, ECO:0000269|PubMed:12130536, ECO:0000269|PubMed:19527706}.
- Molecular Weight
- 50.2 kDa
- UniProt
- P31361
- AlphaFold
- P31361
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