DDIT3 Protein (AA 1-168) (His tag)

Catalog No. ABIN7563183

Product Details

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Protein Type: Recombinant

Protein Characteristics: AA 1-168

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DDIT3 protein is labelled with His tag.

Purpose: Made-to-order recombinant Ddit3 Protein expressed in mammalian cells.

Sequence: MAAESLPFTL ETVSSWELEA WYEDLQEVLS SDEIGGTYIS SPGNEEEESK TFTTLDPASL AWLTEEPGPT EVTRTSQSPR SPDSSQSSMA QEEEEEEQGR TRKRKQSGQC PARPGKQRMK EKEQENERKV AQLAEENERL KQEIERLTRE VETTRRALID RMVSLHQA Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: Ddit3 (DDIT3 Products)

Background: DNA damage-inducible transcript 3 protein (DDIT-3) (C/EBP zeta) (C/EBP-homologous protein) (CHOP) (C/EBP-homologous protein 10) (CHOP-10) (CCAAT/enhancer-binding protein homologous protein) (Growth arrest and DNA-damage-inducible protein GADD153),FUNCTION: Multifunctional transcription factor in endoplasmic reticulum (ER) stress response (PubMed:15601821, PubMed:19752026). Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress (PubMed:15601821, PubMed:19752026). Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes (PubMed:1547942). Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes (PubMed:1547942). Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L (PubMed:12706815, PubMed:15775988, PubMed:21159964, PubMed:14684614, PubMed:19919955). Negatively regulates, expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG) (By similarity). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress (PubMed:22242125, PubMed:23624402). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity (By similarity). Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response (PubMed:16670335). Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling (PubMed:22265908). {ECO:0000250|UniProtKB:P35638, ECO:0000269|PubMed:12706815, ECO:0000269|PubMed:14684614, ECO:0000269|PubMed:1547942, ECO:0000269|PubMed:15601821, ECO:0000269|PubMed:15775988, ECO:0000269|PubMed:16670335, ECO:0000269|PubMed:19752026, ECO:0000269|PubMed:19919955, ECO:0000269|PubMed:21159964, ECO:0000269|PubMed:22242125, ECO:0000269|PubMed:22265908, ECO:0000269|PubMed:23624402}.

Molecular Weight: 19.2 kDa

UniProt: P35639

AlphaFold: P35639

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis