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DPAGT1 Protein (AA 1-410) (His tag)

custom-made DPAGT1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Catalog No. ABIN7563251
  • Target See all DPAGT1 Proteins
    DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-410
    Origin
    • 4
    • 1
    Mouse
    Source
    • 2
    • 2
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This DPAGT1 protein is labelled with His tag.
    Purpose
    Predefined custom protein recombinant Dpagt1 Protein expressed in mammalian cells.
    Sequence
    MWAFPELPLP LPLLVNLIGS LLGFVATVTL IPAFRSHFIA ARLCGQDLNK LSQQQIPESQ GVISGAVFLI ILFCFIPFPF LNCFVEEQCK AFPHHEFVAL IGALLAICCM IFLGFADDVL NLRWRHKLLL PTAASLPLLM VYFTNFGNTT IVVPKPFRWI LGLHLDLGIL YYVYMGLLAV FCTNAINILA GINGLEAGQS LVISASIIVF NLVELEGDYR DDHIFSLYFM IPFFFTTLGL LYHNWYPSRV FVGDTFCYFA GMTFAVVGIL GHFSKTMLLF FMPQVFNFLY SLPQLFHIIP CPRHRMPRLN AKTGKLEMSY SKFKTKNLSF LGTFILKVAE NLRLVTVHQG ESEDGAFTEC NNMTLINLLL KVFGPIHERN LTLLLLLLQV LSSAATFSIR YQLVRLFYDV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Specificity
    If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
    Characteristics
    Key Benefits:
    • Predefined custom protein - from design to production - by highly experienced protein experts.
    • Protein expressed in mammalian cells and purified in one-step affinity chromatography
    • The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    If you are not interested in a full length protein, please contact us for individual protein fragments.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
    Purity
    > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
    Grade
    custom-made
    Top Product
    Discover our top product DPAGT1 Protein
  • Application Notes
    We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    12 months
  • Target
    DPAGT1 (Dolichyl-Phosphate (UDP-N-Acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P Transferase) (DPAGT1))
    Alternative Name
    Dpagt1 (DPAGT1 Products)
    Background
    UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC 2.7.8.15) (GlcNAc-1-P transferase) (G1PT) (GPT) (N-acetylglucosamine-1-phosphate transferase),FUNCTION: Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis in N-linked protein glycosylation pathway: transfers GlcNAc-1-P from UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol. {ECO:0000250|UniProtKB:Q9H3H5}.
    Molecular Weight
    46.4 kDa
    UniProt
    P42867
    AlphaFold
    P42867
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