SIPA1 Protein (AA 1-1037) (His tag)

Catalog No. ABIN7563270

Recombinant SIPA1 protein expressed in HEK-293 Cells.

Quick Overview for SIPA1 Protein (AA 1-1037) (His tag) (ABIN7563270)

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1037

Purification tag / Conjugate: This SIPA1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Sipa1 Protein expressed in mammalian cells.

Sequence: MWAGGVGSPR RGMAPAPTDD LFARKLRQPA RPPLTPNTFE PRPARGPLLR SGSDAGEVRP PTPASPRARA HSHEDASRPA ATPTRLFTDP LALLGLPAEE PEPTFPPVLE PRWFAHYDVQ SLLFDWAPRP RGTGSHTEAN SGTLAEGQTT TSDLLLGAPG FVSELGGEGE LGLGGPISPP VPPALPNAAV SVLEEPQTRT TTYSLEHADL GAGYYRKYFY GKEHQNFFGL DEALGPVAVS LRREEKEGSG GGTLHSYRVI VRTTQLRTLR GTISEDALPP GPPSVSPRKL LEHVAPRLSP TCLRLGSASP KVPRQLLTLD EQVLSFQRKG GILYCRAGQG SEEEMYNNQE AGAAFMQFLT LLGDVVRLKG FESYRAQLDT KTDSTGTHSL YTTYQDHEIM FHVSTMLPYT PNNQQQLLRK RHIGNDIVTI VFQEPGSKPF CPTTIRSHFQ HVFLVVRAHA PCTPHTSYRV AVSRTQDTPA FGPALPEGGG PFAANADFRA FLLAKALNGE QAAGHARQFH AMATRTRQQY LQDLATNEVT TTSLDSASRF GLPSLGGRRR ATPRSPGADV QAAGALMWGV RAAPGARVAA GAETSGPDDA EVPCLLGISA ETLVLVAPRD GRVVFNCACR DVLAWTFSEH QLDLYHGRGE AITLRLDGAP GQAVGEVVAR LQLVSRGCET RELALPRDGQ GRLGFEVDAE GFITHVERFT FAETTGLRPG ARLLRVCGQT LPKLGPETAA QMLRSAPKVC VTVLPPDESG RPRRSFSELY MLSLKEPSRR GGPEPVQDET GKLVILPPTK QLLHFCLKDS SSPPGPGDLT EERTEFLRTH NSLSSGSSLS DEAPVLPNTT PDLLLVTTAN PSAPGTDRET PPSQDQSGSP SSHEDTSDSG PELRASILPR TLSLRNSISK IMSEAGSETL EDEWQSISEI ASTCNTILES LSREGQPISE SGDPKEALKC DSEPEPGSLS EKVSHLESML WKLQEDLQRE KADRAALEEE VRSLRHNNQR LLAESESAAT RLLLASKHLG APTTDLA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Alternative Name: Sipa1

Background: Signal-induced proliferation-associated protein 1 (Sipa-1) (GTPase-activating protein Spa-1),FUNCTION: GTPase activator for the nuclear Ras-related regulatory proteins Rap1, Rsr1 and Ran in vitro, converting them to the putatively inactive GDP-bound state. Affects cell cycle progression. {ECO:0000269|PubMed:7799964}.

Molecular Weight: 112.1 kDa

UniProt: P46062

AlphaFold: P46062

Pathways: Response to Water Deprivation