RFX2 Protein (AA 1-717) (His tag)

Catalog No. ABIN7563312

Product Details

Target: RFX2 (Regulatory Factor X 2 (RFX2))

Protein Type: Recombinant

Protein Characteristics: AA 1-717

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RFX2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rfx2 Protein expressed in mammalian cells.

Sequence: MQNSEGGADS PASVALRPAA QPMPASPQRV LVQAAGSTPK GTPMQTLTLP RVQPVPPQVQ HVYPAQVQYV EGGDAVYANG AIRAAYAYNP DPQLYAPSSA ASYFETPGGT QVTVAASSPP AVPSHGMVGI TMDVSGTPIV SGAGAYLIHG GMDGTRHSLA HTARSSPATL EMAIETLQKS EGLAPHKGGL LNSHLQWLLD NYETAEGVSL PRSSLYNHYL RHCQEHKLEP VNAASFGKLI RSVFMGLRTR RLGTRGNSKY HYYGIRLKPD SPLNRLQEDT QYMAMRQQPT HQKPRYRPAQ KSDSLGDGSA HSNMHGMPDQ AMATQGQHHQ QYIDVSHVFP EFPAPDLGST LLQESVTLHD VKALQLVYRR HCEATLDVVM NLQFQYIEKL WLSFWNCKAT SSDSCASLPA SDEDPEVTLL PKEKLISLCK CEPILQWMRS CDHILYQTLV ETLIPDVLRP VPSSLTQAIR NFAKSLEGWL INAMSGFPQQ VIQTKVGVVS AFAQTLRRYT SLNHLAQAAR AVLQNTSQIN QMLSDLNRVD FANVQEQASW VCQCEESLVQ RLEHDFKVTL QQQSSLDQWA SWLDNVVTQV LKQHSGSPSF PKAARQFLLK WSFYSSMVIR DLTLRSAASF GSFHLIRLLY DEYMFYLVEH RVAQATGETP IAVMGEFNDL ASLSLTLLDK EDIGDGHSSE ADVDGRSLGE PLVKRERSDP SHPLQGI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RFX2 (Regulatory Factor X 2 (RFX2))

Alternative Name: Rfx2 (RFX2 Products)

Background: DNA-binding protein RFX2 (Regulatory factor X 2),FUNCTION: Transcription factor that acts as a key regulator of spermatogenesis (PubMed:26248850, PubMed:26162102, PubMed:26853561). Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function (PubMed:26162102, PubMed:26853561). Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters (PubMed:15229132, PubMed:26162102). Probably activates transcription of the testis-specific histone gene H1-6 (PubMed:15229132). {ECO:0000269|PubMed:15229132, ECO:0000269|PubMed:26162102, ECO:0000269|PubMed:26248850, ECO:0000269|PubMed:26853561}.

Molecular Weight: 79.2 kDa

UniProt: P48379

AlphaFold: P48379