RFX3 Protein (AA 1-749) (His tag)
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- Target See all RFX3 Proteins
- RFX3 (Regulatory Factor X 3 (RFX3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-749
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RFX3 protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Rfx3 Protein expressed in mammalian cells.
- Sequence
- MQTSETGSDT GSTVTLQTSV ASQAAVPTQV VQQVPVQQQV QQVQTVQQVQ HVYPAQVQYV EGSDTVYTNG AIRTTTYPYT ETQMYSQNTG GNYFDTQGSS AQVTTVVSSH SMVGTGGIQM GVTGGQLISS SGGTYLIGNS MENSGHSVTH TTRASPATIE MAIETLQKSD GLSTHRSSLL NSHLQWLLDN YETAEGVSLP RSTLYNHYLR HCQEHKLDPV NAASFGKLIR SIFMGLRTRR LGTRGNSKYH YYGIRVKPDS PLNRLQEDMQ YMAMRQQPMQ QKQRYKPMQK VDGVADGFTG SGQQTGTSVE QTVIAQSQHH QQFLDASRAL PEFGEVEISS LPDGTTFEDI KSLQSLYREH CEAILDVVVN LQFSLIEKLW QTFWRYSPST PADGTTITES SNLSEIESRL PKAKLITLCK HESILKWMCN CDHGMYQALV EILIPDVLRP IPSALTQAIR NFAKSLEGWL SNAMNNIPQR MIQTKVAAVS AFAQTLRRYT SLNHLAQAAR AVLQNTSQIN QMLSDLNRVD FANVQEQASW VCQCDDNMVQ RLETDFKMTL QQQSTLEQWA AWLDNVMMQA LKPYEGRPSF PKAARQFLLK WSFYSSMVIR DLTLRSAASF GSFHLIRLLY DEYMFYLVEH RVAQVTGETP IAVMGEFGDL NAVSPGNLDK DEGSEVESET DEDLDDSSEP RAKREKTELS QAFPVGCMQP VLESAVQPSL LNPLHSEHIV TSTQTIRQCS ATGNTYTAV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Regulatory Factor X 3 (RFX3) (AA 1-749) protein (His tag)
custom-made RFX3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Regulatory Factor X 3 (RFX3) (AA 1-749) protein (GST tag)RFX3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Regulatory Factor X 3 (RFX3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)RFX3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- RFX3 (Regulatory Factor X 3 (RFX3))
- Alternative Name
- Rfx3 (RFX3 Products)
- Background
- Transcription factor RFX3 (Regulatory factor X 3),FUNCTION: Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells (PubMed:15121860, PubMed:19671664). Also required for ciliated ependymal cell differentiation (PubMed:16930429). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells (PubMed:17229940). Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (By similarity). Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. {ECO:0000250|UniProtKB:P48380, ECO:0000269|PubMed:15121860, ECO:0000269|PubMed:16930429, ECO:0000269|PubMed:17229940, ECO:0000269|PubMed:19671664}.
- Molecular Weight
- 83.5 kDa
- UniProt
- P48381
- AlphaFold
- P48381
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