LHX3 Protein (AA 1-400) (His tag)

Catalog No. ABIN7563363

Product Details

Target: LHX3 (LIM Homeobox 3 (LHX3))

Protein Type: Recombinant

Protein Characteristics: AA 1-400

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This LHX3 protein is labelled with His tag.

Purpose: Made-to-order recombinant Lhx3 Protein expressed in mammalian cells.

Sequence: MLLEAELDCH RERPGAPGAS ALCTFSRTPE IPMCAGCDQH ILDRFILKAL DRHWHSKCLK CSDCHVPLAE RCFSRGESVY CKDDFFKRFG TKCAACQLGI PPTQVVRRAQ DFVYHLHCFA CVVCKRQLAT GDEFYLMEDS RLVCKADYET AKQREAEATA KRPRTTITAK QLETLKSAYN TSPKPARHVR EQLSSETGLD MRVVQVWFQN RRAKEKRLKK DAGRQRWGQY FRNMKRSRGS SKSDKDSIQE GQDSDAEVSF TDEPSMADMG PANGLYSSLG EPAPALGRPV GGLGSFTLDH GGLTGPEQYR ELRPGSPYGI PPSPAAPQSL PGPQPLLSSL VYPDTNLSLV PSGPPGGPPP MRVLAGNGPS SDLSTESSSG YPDFPASPAS WLDEVDHAQF Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: LHX3 (LIM Homeobox 3 (LHX3))

Alternative Name: Lhx3 (LHX3 Products)

Background: LIM/homeobox protein Lhx3 (LIM homeobox protein 3) (Homeobox protein LIM-3) (Homeobox protein P-LIM),FUNCTION: Transcription factor (PubMed:18539116, PubMed:10593900, PubMed:12150931). Recognizes and binds to the consensus sequence motif 5'-AATTAATTA-3' in the regulatory elements of target genes, such as glycoprotein hormones alpha chain CGA and visual system homeobox CHX10, positively modulating transcription, transcription can be co-activated by LDB2 (PubMed:18539116, PubMed:10593900, PubMed:9192866). Synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (PubMed:7708713). Required for the establishment of the specialized cells of the pituitary gland and the nervous system (By similarity). Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1 (PubMed:12150931, PubMed:18539116). {ECO:0000250|UniProtKB:Q9UBR4, ECO:0000269|PubMed:10593900, ECO:0000269|PubMed:12150931, ECO:0000269|PubMed:18539116, ECO:0000269|PubMed:7708713, ECO:0000269|PubMed:9192866}.

Molecular Weight: 44.0 kDa

UniProt: P50481

AlphaFold: P50481