Six5 Protein (AA 1-719) (His tag)

Catalog No. ABIN7563513

Recombinant Six5 protein expressed in HEK-293 Cells.

Quick Overview for Six5 Protein (AA 1-719) (His tag) (ABIN7563513)

Target: Six5 (SIX5) (Homeobox protein SIX5 (SIX5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-719

Purification tag / Conjugate: This Six5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Six5 Protein expressed in mammalian cells.

Sequence: MATSPAEPSA GPAARGEAAA ATEEQEEEAR QLLQTLQAAE GEAAAAGAGD AAAAADSGSP SGPGSPRETV TEVPTGLRFS PEQVACVCEA LLQAGHAGRL SRFLGALPPA ERLRGSDPVL RARALVAFQR GEYAELYQLL ESRPFPAAHH AFLQDLYLRA RYHEAERARG RALGAVDKYR LRKKFPLPKT IWDGEETVYC FKERSRAALK ACYRGNRYPT PDEKRRLATL TGLSLTQVSN WFKNRRQRDR TGTGGGAPCK SESDGNPTTE DESSRSPEDL ERGVASMAAE APAQSSIFLA GATSPATCPA SSSILVNGSF LAASSPPAVL LNGSPVIINS LALGENSSLG PLLLTGGSAP QPQPSLQGVS EAKNSLVLDP QTGEVRLDEA QSEAPETKGV HGTTGEEIPG ALPQVVPGPP PASTFPLTPG AVPAVAAPQV VPLSPSSGYP TGLSPTSPRL NLPQVVPTSQ VVTLPQAVGP LQLLAAGPGS PVKVAAAAGP TNVHLINSSV GVTALQLPSS TAPGNFLLAN PVSGSPIVTG VAVQQGKIIL TATFPTSMLV SQVLPPAPSL ALPLKQEPAI TVPEGALPVG PSPTLPEGHT LGPISTQPLP PASVVTSGTS LPFSPDSSGL LSSFSAPLPE GLMLSPAAVP VWPAGLELST GVEGLGTQAT HTVLRLPDPD PQGLLLGATT GTEVDEGLEA EAKVLTQLQS VPVEEPLEL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Six5 (SIX5) (Homeobox protein SIX5 (SIX5))

Alternative Name: Six5

Background: Homeobox protein SIX5 (DM locus-associated homeodomain protein homolog) (Sine oculis homeobox homolog 5),FUNCTION: Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter (By similarity). Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3. {ECO:0000250, ECO:0000269|PubMed:10490620}.

Molecular Weight: 73.7 kDa

UniProt: P70178

AlphaFold: P70178