MCM10 Protein (AA 1-885) (His tag)

Catalog No. ABIN7563621

Recombinant MCM10 protein expressed in HEK-293 Cells.

Quick Overview for MCM10 Protein (AA 1-885) (His tag) (ABIN7563621)

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-885

Purification tag / Conjugate: This MCM10 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Mcm10 Protein expressed in mammalian cells.

Sequence: MDVEEDDLCL LTSLLEENEA VLPCSSEKDK SLSLGDGDPD EFDELFDADG DGESYTEEAG SGEEGKTGNQ EERLATLFGD VEDLTDDEVA TSKVGNSGPP PAPSQEKTSE ELQDELKKLQ EQMKSLQEQL KAASIKQPPG TAPLQEPPDS SLQPLLKEKR IRRIQESACF SAELDVPTLP KAKRVARKPK TPAESSSRMR TPAQPLQVSS SFLEPNHSSS SRSSTPSPQA VPGNKCSRTI RNQNTVSPGN SGDRPQQVSQ VSVEAFSGLR LRRPRVSSTE MSRKMAGRKL IRLPQIKEKM ATENLEETDW VTFGVILRKV TPQSATSGQT FSIWKLNDLH DLTQCVSLFL FGDVHKDLWK TEQGTVIGLL NANPMKPKDG LKEVCLSIDH PQKVLIMGEA MDLGACKAKK KNGEPCTQTV NLHDCEYCQY HIQAQYKKLS AKRTDLQSTF SGGRIPKKFR KGTSLKERLC QDGFYYGGVS SESFAASRAA AIAPKKKVQT TLTNLVVRGT NSIIQETKQK LGIPQKSLSC SEEFRELMAL PTFGARNLQK HLARAKASGS SKPAIQSISA SALLKQQKQQ MLEMRKRRSE DIQKRFLQSS SEVQSPAVPS SSRQAAAQSP RTGAEFPRLE GTATPRMPKL GRGISEGDDV LFFDDSPPPR PKLSAAAEAK KLAAIAKLRA KGQILTKVDP NNTVRKQMDG RAMLGVKERV ENSNTVSPEE ELEPARKKRR EQLAYLESEE FQKILKAKSK HTDILKEAEA ELQKSYFEPL VKKEQMEEKM RATREVKCRV VTCRTCTYTH FKPLETCVSE QHNLHWHDGV KRFFKCPCGN RTISLDKLPN KHCRNCGLYK WERDGMLKEK TGPKIGGETL LPRGEEHAKF LNSLK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Alternative Name: Mcm10

Background: Protein MCM10 homolog,FUNCTION: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability, acts as a direct target of transcriptional repression by ZBTB38 (By similarity). {ECO:0000250|UniProtKB:Q7L590}.

Molecular Weight: 98.4 kDa

UniProt: Q0VBD2

AlphaFold: Q0VBD2

Pathways: Mitotic G1-G1/S Phases, DNA Replication