MYO3B Protein (AA 1-1305) (His tag)

Catalog No. ABIN7563642

Recombinant MYO3B protein expressed in HEK-293 Cells.

Quick Overview for MYO3B Protein (AA 1-1305) (His tag) (ABIN7563642)

Target: MYO3B (Myosin IIIB (MYO3B))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1305

Purification tag / Conjugate: This MYO3B protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Myo3b Protein expressed in mammalian cells.

Sequence: MMLGLESLPD PMETWEIIET IGKGTYGKVY KVANKRDGSL AAVKVLDPVS DMDEEIEAEY NILQFLPSHP NVVKFYGMFY KADRCVGGQL WLVLELCNGG SVTELVKGLL RCGKRLDEAV ISYILYGALL GLQHLHCHRI IHRDVKGNNI LLTTEGGVKL VDFGVSAQLT STRLRRNTSV GTPFWMAPEV IACEQQYDSS YDARCDVWSL GITAIELGDG DPPLFEMHPV KMLFKIPRNP PPTLLHPDSW CEEFNHFISQ CLIKDFEKRP SVTHLLDHPF IKGTQGKVLC LQKQLAKVLQ DQKHRNPVAK TRHERMHTGR PHRVEDAGKC CLEDDLVNLE VLDEDTIIYW LQKRYADALI YTYVGDILIA LNPFQNLSIY SPQFSRLYHG VKRSSNPPHI FASADNAYQC LVTFSKDQCI VISGESGSGK TESAHLIVQH LTFLGKADNQ TLRQKILQVN SLVEAFGNAR TAINDNSSRF GKYLEMMFTP TGAVMGARIS EYLLEKSRVI QQAAGEKNFH IFYYIYAGLY HQKKLAEFRL PEEKPPRYIA GETERVMQDI TSKESYRTQF EAIQHCFKII GFADKEVHSV YRILAGILNI GSIEFAAISS QHQTDKSEVP NPEALENAAC VLCISSEELQ EALTSHCVVT RGETIVRANT VDRAEDVRDA MSKALYGRLF SWIVNRINTL LQPDKNICSA EDRMNVGILD IFGFEDFQRN SFEQLCINIA NEQIQYYFNQ HVFALEQMEY KNEGVDAVLV QYEDNRPLLD MFLQKPLGLL ALLDEESRFP QGTDQTLVDK FEDNLRCKFF WRPKGVELCF GIQHYAGPVL YDASGVLEKN RDTLPADVVV VLRTSENKLL QQLFSIPLTK TGNLAQTRAK ITASSRSLPP HFSAGRAKVD TLEVIRHPEE TTNMKRQTMA SYFRYSLMDL LSKMVVGQPH FIRCIKPNDD RKALQFSQDR VLAQLRSTGI LETVSIRRQG YSHRIFFEEF VKRYYYLAFR AHQTPPANKE SCVAILEKSR LDHWVLGKTK VFLKYYHVEQ LNLLLREVMG RVVMLQAYTK GWLGARRYKR AKEKREKGAI TIQSAWRGYD ARRKLKQRSR RRSESEAHIH TVLQTTPDQK YCPDSGGESN RGHEETSRNC PAEADTDGHP QAQSPPTGCD VTSGHADTAA GYTVAELSVA GTDVSPSLVY HTASAHQRLS PCEDSLKPGS EEGLSQKQRA PRRRCQQPKM LSSPEDTMYY NQLNGTLEYQ GSQRKPRKLG QIKVLDGEDQ YYKCLSPGAC APEETHSVHP FFFSSSPRED PFAQH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MYO3B (Myosin IIIB (MYO3B))

Alternative Name: Myo3b

Background: Myosin-IIIb (EC 2.7.11.1),FUNCTION: Probable actin-based motor with a protein kinase activity (By similarity). Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646). Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:22264607). {ECO:0000250|UniProtKB:Q8WXR4, ECO:0000269|PubMed:22264607, ECO:0000269|PubMed:26754646}.

Molecular Weight: 148.1 kDa

UniProt: Q1EG27

AlphaFold: Q1EG27