RNF40 Protein (AA 1-1001) (His tag)
custom-made
RNF40
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all RNF40 Proteins
- RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1001
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RNF40 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Rnf40 Protein expressed in mammalian cells.
- Sequence
- MSGLSNKRAA GDGGSGPPEK KMNREEKTTT TLIEPIRLGG ISSTEEMDSK VLQFKNKKLA ERLEQRQACE DELRERIEKL EKRQATDDAT LLIVNRYWAQ LDETVEALLQ CYENQRELSS GTEVPGCQEG LTRDVIPRPD PGTSDLREPL PVQFRAPLSE PALAFVVALG ASSCEEVELQ LQGRMEFSKA AVSRVVEASD RLQRQVEELC QRVYSRGDSE APGEVARVRT RELGRENRRL QDLATQLQEK HHRISLEYSE LQDKVTSTET KVLEMETTVE DLQWDIEKLR KREQKLNKHL AEALEQLNSG YYVSGSSTGF QGGQITLSMQ KFEMLNAELE ENQELANSRM AELEKLQAEL QGAVRTNERL KVALRSLPEE VVRETGEYRM LQAQFSLLYN ESLQVKTQLD EARGLLLASK NSHLRHIEHM ESDELGLQKK LRTEVIQLED TLAQVRKEYE MLRIEFEQNL AANEQAGPIN REMRHLISSL QNHNHQLKGD AQRYKRKLRE VQAEIGKLRA QASGSSHCIP TLSHPDDPGL NALAPGKEDS GPGPGGTPDC KKEMALLAGA TSATSSIKKE ELVSSEDDAQ ALTPVTQGLP SRGREPEARP KRELREREGP SLGPPPAAST LSRADREKAK VEEAKRKESE LLKGLRAELK KAQESQKEMK LLLDMYKSAP KEQRDKVQLM AAERKAKAEV DELRSRIREL EERDRRESKK IADEDALRRI RQAEEQIEHL QRKLGATKQE EEALLSEMDV TGQAFEDMQE QNGRLLQQLR EKDDANFKLM SERIKANQIH KLLREEKDEL GEQVLGLKSQ VDAQLLTVQK LEEKERALQG SLGGVEKELT LRSQALELNK RKAVEAAQLA EDLKVQLEHV QTRLREIQPC LAESRAAREK ESFNLKRAQE DISRLRRKLE KQRKVEVYAD ADEILQEEIK EYKARLTCPC CNTRKKDAVL TKCFHVFCFE CVRGRYEARQ RKCPKCNAAF GAHDFHRVYI S Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product RNF40 Protein
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Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40) (AA 1-1001) protein (His tag)
custom-made RNF40 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))
- Alternative Name
- Rnf40 (RNF40 Products)
- Background
- E3 ubiquitin-protein ligase BRE1B (BRE1-B) (EC 2.3.2.27) (RING finger protein 40) (RING-type E3 ubiquitin transferase BRE1B),FUNCTION: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B, reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. {ECO:0000250|UniProtKB:O75150}.
- Molecular Weight
- 114.0 kDa
- UniProt
- Q3U319
- AlphaFold
- Q3U319
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