SSH2 Protein (AA 1-1423) (His tag)

Catalog No. ABIN7563848

This Recombinant SSH2 protein is expressed in HEK-293 Cells.

Quick Overview for SSH2 Protein (AA 1-1423) (His tag) (ABIN7563848)

Target: SSH2 (Slingshot Homolog 2 (SSH2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1423

Purification tag / Conjugate: This SSH2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ssh2 Protein expressed in mammalian cells.

Sequence: MALVTVQRSP TPSTTSSPCA SEADSGEEEC RSQPRSISES FLTVKGAALF LPRGNGSSTP RVSHRRNKHA GDLQQHLQAM FILLRPEDNI RLAVRLESTY QNRTRYMVVV STNGRQDTEE SIVLGMDFSS NDSSTCTMGL VLPLWSDTLI HLDGDGGFSV STDNRVHIFK PVSVQAMWSA LQSLHKACEV ARMHNYYPGS LFLTWVSYYE SHINSDQSSV NEWNAMQDVQ SHRPDSPALF TDIPTERERT ERLIKTKLRE IMMQKDLENI TSKEIRTELE MQMVCNLREF KEFIDNEMIV ILGQMDSPTQ IFEHVFLGSE WNASNLEDLQ NRGVRYILNV TREIDNFFPG VFEYHNIRVY DEEATDLLAY WNDTYKFISK AKKHGSKCLV HCKMGVSRSA STVIAYAMKE YGWNLDRAYD YVKERRTVTK PNPSFMRQLE EYQGILLASK QRHNKLWRSH SDSDLSDHHE PICKPGLELN KKEMTTSADQ IAEVKTVENL AAMPTVFMEH VVPQDANQKG LHTKERVICL EFSSQEFRAG QIEDELNLND INGCSSGCCL SESKLPLDNC HASKALLQPG QAPDIANKFP DLAVEDLETD ALKADMNVHL LPMEELTSRL KDLPMSPDLE SPSPQASCQA AISDFSTDRI DFFSALEKFV ELSQETRSRS FSHSRIEELG GGRSEGCRLS VIEVAASEMA ADDQRSSSLS NTPHASEESS VDEDQSKAIT ELVSPDIIMQ SHSENAISVK EIVTEIESIS QGVGQVQLKG DILSNPCHTP KKSTIHELPL ERVPAPESKP GHWEQDESFC SVQPELARDS GKCAPEEGCL TTHSSTADLE EEEPVEGEHD WGPGMHSGAK WCPGSVRRAT LEFEERLRQE QENHGTASAG PTLSNRKNSK NDSSVADLMP KWKSDETTPE HSFFLKEAEP SKGKGKCSGS EAGSLSHCER NPTMPDCELL EHHSLPAPQD CLGSDSRSKK QEGDLKKQRA VVPNQECDTQ AILLPLPKKI EIIEYTPTVT SLGHTEPGGE ATPSKEGEKQ GLRKVKMEQS ITMFCALDEN LNRTLEPSQV SLHPQVLPLP HSSSECDRPA DPNPMLSSPQ DKGDCPSTPF KTAAPFVSCS TQGASFSLDY LLPHSVVHLE GCTEQSSATD NELSPEQASW EDSRGHFLSS GSGMAHTSSP LTNEDLSLIN KLGDSVGVLQ KKLDPSPEAC RIPHSSSSEN IRDLSHSRGV VKEHAKEIES RVIFQAGFSK TSQMKRSASL AKLGYLDLCK DYLPDRELVS SESPHLKLLQ PFLRTDSGMH ALMAHEPSES AGAQQNPQPT KYSVEQLKTS ECIVQSKPVE RPSVQYAKEF GYSQQCLLPK ARPELTSSEG GLPLLQTQGL QYTGPSPGLA VAPRQQHGRT HPLRRLKRAN DKKRTTNPFY NTM Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SSH2 (Slingshot Homolog 2 (SSH2))

Alternative Name: Ssh2

Background: Protein phosphatase Slingshot homolog 2 (EC 3.1.3.16) (EC 3.1.3.48) (SSH-like protein 2) (SSH-2L) (mSSH-2L),FUNCTION: Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein (PubMed:14531860). Required for spermatogenesis (PubMed:36942942). Involved in acrosome biogenesis, probably by regulating cofilin-mediated actin cytoskeleton remodeling during proacrosomal vesicle fusion and/or Golgi to perinuclear vesicle trafficking (PubMed:36942942). {ECO:0000269|PubMed:14531860, ECO:0000269|PubMed:36942942}.

Molecular Weight: 158.2 kDa

UniProt: Q5SW75

AlphaFold: Q5SW75