RAD17 Protein (RAD17) (AA 1-688) (His tag)

Catalog No. ABIN7564065

Recombinant RAD17 protein expressed in HEK-293 Cells.

Quick Overview for RAD17 Protein (RAD17) (AA 1-688) (His tag) (ABIN7564065)

Target: RAD17

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-688

Purification tag / Conjugate: This RAD17 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rad17 Protein expressed in mammalian cells.

Sequence: MSETFLRPKV SSTKVTDWVA PAFDDFEANT AITTITASSL TFSNSSHRRK YLPSTLESNR LSARKRGRLS LEQTHGLETS RERLSDNEPW VDKYKPETQH ELAVHKKKIE EVETWLKAQV LEVKPKQGGS VLLITGPPGC GKTTTIKILS KELGIQVQEW VNPILPDFQK DDYKELLSLE SNFSVVPYQS QIAVFNDFLL RATKYSKLQM LGDDLTTDKK IILVEELPNQ FYRDPNALHE ILRKHVQIGR CPLVFIVSDS VSGDNNQRLL FPRNIQEECS VSNISFNPVA PTIMMKFLNR IVTIEASKNG EKIIVPNKTS LELLCQGCSG DIRSAINSLQ FSSSKGENSS WSKKKRMSLK SDAAISKSKQ KKKHNSTLEN QEIQAIGGKD VSLFLFRALG KILYCKRAPL TELDSPRLPA HLSEHDRDTL LVQPEEIVEM SHMPGDFFNL YLHQNYIDFF AEVDDLVPAS EFLSFADILG GDWNTRSLLR EYSTSVATRG VMHSNKARGF AHCQGGSSFR PLHKPQWFLI QKKYRENCLA AKALFVDFCL PALCLQTQLL PYLALLTIPM RNKAQISFIQ DVGRLPLKRS FGRLKMEALT DRELGLIDPD SGDESPHSGG QPAQEAPGEP AQAAQNADPE TWSLPLSQNS GSDLPASQPQ PFSSKVDMEE EEEEEEDIII EDYDSEET Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RAD17

Alternative Name: Rad17

Background: Cell cycle checkpoint protein RAD17,FUNCTION: Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage (By similarity). Has a weak ATPase activity required for binding to chromatin (By similarity). Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation (By similarity). May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination (PubMed:15297881). {ECO:0000250|UniProtKB:O75943, ECO:0000269|PubMed:15297881}.

Molecular Weight: 77.4 kDa

UniProt: Q6NXW6

AlphaFold: Q6NXW6