PAXIP1 Protein (AA 1-1056) (His tag)

Catalog No. ABIN7564074

Product Details

Target: PAXIP1 (PAX Interacting (With Transcription-Activation Domain) Protein 1 (PAXIP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1056

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PAXIP1 protein is labelled with His tag.

Purpose: Made-to-order recombinant Paxip1 Protein expressed in mammalian cells.

Sequence: MSEPAPEVPE ELFREVKYYA VGDIDPQVIQ LLKAGKAKEV SYNALASHII SEDGDNPEVG EAREVFDLPV VKPSWVTLSV QCGALLPVNG FSPESCQIFF GLTACLSQVS SEDRSALWAL VTFHGGSCQL NLNKKCTHLI VPEPKGEKYE RAVKRTSIKI VTPDWVLDCV SEKRRKDEAF YHPRLIIYEE EEEEEEEGDN EEQDSQNEGS TEKSSVASSA VASPAEQPCS PKPRAEVSKG ELMFDDSSDS SPEKQERSLN WAPAEAPPLN TAQRRLPQGK GPGLINLCAN VPPVPGDILP PDMRGNLMAP GQNLQNSERS EILGTWSPAV RTLRNITNNA DIQQINRPSN VAHILQSLSA PTKSLEQQVA RGQQGHPNAS AVLFGQAKGA PETHVLQQHH PPQQPQQQHP ALHLQPQIMQ LQQQQQQQQQ QQQQPQPYPQ PPSHQFPQQV HQHQFSQQQL QFPQQPLHPQ QQLHRPQQQL QPFQQQHALQ QQLHQLQQQQ LQHHQLAQLQ QQQQQQHNLL QQQQQQQQLQ RLQQQQQMQN QAAHLSQASQ ALQHQVLPQQ PLQLSLQPPP QQQQQQQLFG HDPAVEIPEE SFLLGCVFAI ADYPEQMSDK QLLATWKRII QAHGGTVDPT FTSRCTHLLC ASQVSSMYTQ ALRERKRCVT AHWLNTVLKK KKLMPPHRAL HFPVAFPPGG KPCSQHIISV TGFVDNDRDD LKLMAYLAGA KYTGYLCRSN TVLICKEPSG LKYEKAKEWR IPCVNAQWLG DILLGNFEAL RQVQYSRYTA FNMPDPFVPT PHLVLGLLDA WRTPVKVTAE LLMGVRLPPK LKPNEVANIQ PSSKRARIED LPPPTKKLTP ELTPLVLFTG FEPVQVQQYI KKLYILGGEV AECTKKCTHL IASKVTRTVK FLTAISVVKH IVTPDWLEEC FKRQTFIDEQ NYILRDAEAE VLFSFSLEES LKRAHVSPLF KTKYFYITPG ICPSLATMKA IVECAGGKVL AKQPSFRKLM EHKQNKSLSE IILISCENDL HLCREYFARG IDVHNAEFVL TGVLTQTLDY ESYKFN Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PAXIP1 (PAX Interacting (With Transcription-Activation Domain) Protein 1 (PAXIP1))

Alternative Name: Paxip1 (PAXIP1 Products)

Background: PAX-interacting protein 1 (PAX transactivation activation domain-interacting protein),FUNCTION: Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes such as the MLL2/MLL3 complex. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires Rnf8 and Ube2n. Recruits Tp53bp1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by Atm. Together with Tp53bp1 regulates Atm association (By similarity). Proposed to recruit Pagr1 to sites of DNA damage and the Pagr1:Paxip1 complex is required for cell survival in response to DNA damage independently of the MLL2/MLL3 complex. However, this function has been questioned (PubMed:19124460, PubMed:26744420). Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and Rad51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as Pax2. Associates with gene promoters that are known to be regulated by Kmt2d/Mll2 (By similarity). During immunoglobulin class switching in activated B-cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus, this function appears to involve the recruitment of MLL-containing HMT complexes. Conflictingly, its function in transcriptional regulation during immunoglobulin class switching is reported to be independent of the MLL2/MLL3 complex (PubMed:20671152, PubMed:26744420). {ECO:0000250, ECO:0000269|PubMed:10908331, ECO:0000269|PubMed:12588986, ECO:0000269|PubMed:17925232, ECO:0000269|PubMed:19124460, ECO:0000269|PubMed:19414588, ECO:0000269|PubMed:20671152, ECO:0000269|PubMed:26744420}.

Molecular Weight: 119.3 kDa

UniProt: Q6NZQ4

AlphaFold: Q6NZQ4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response