DGKQ Protein (AA 1-934) (His tag)
custom-made
DGKQ
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target See all DGKQ products
- DGKQ (Diacylglycerol Kinase, theta 110kDa (DGKQ))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-934
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DGKQ protein is labelled with His tag.
- Purpose
- Made-to-order recombinant Dgkq Protein expressed in mammalian cells.
- Sequence
- MAAAAEPGAR TWPGSGSPRL GSPAGSPVLG ISGRTRPGSG PERTSRAIGS AAPGHSFRKV TLTKPTFCHL CSDFIWGLAG FLCDVCNFMS HEKCLKQVKT PCTSIAPSLV RVPVAHCFGS LGLYKRKFCV VCRKSLEVPA FRCEVCELHV HPDCVPFACS DCRQCHQDGQ QDYDTYHHHW REGNLPSGAR CEVCRKTCGS SDVLAGVRCE WCGVQAHSVC STALAPECTF GRLRSMVLPP SCVRLLSRNF SKMHCFRIPE TMVLELGDGD DGVDGSAAIG TGREVLTATE STKQTLKIFD GNDSMRKNQF RLVTVSRLAR NEEVMEAALR AYYISEDPKD FQLQALPLSG NAQALGKAGT TEEEASKGSC PRDSVPEAWV IRSLPRTQEI LKIYPGWLKV GVAYVSIRVN SQSTARSVVQ EVLPLFGQQV EDKERFQLIE VLMSSRQVQR TVLADEEPLL DRLWDIRQTS VRQVSQTRFY VAETRATAPR VSLFVGGLPP GLSPQDYSNL LHEAMATKAA VVSVSHVYSL QGAVILDVTC FAEAERLYML ARDTAVHGRP LTALVLPDVL HTKLPPDCCP LLVFVNPKSG GLKGRELLCS FRKLLNPHQV FELTNGGPLP GFHLFSQVPS FRVLVCGGDG TVGWVLAALE ETRRHLACPE PSVAILPLGT GNDLGRVLRW GAGYSGEDPF SVLVSVDEAD AVLMDRWTIL LDAHEIDSTE NNVVETEPPK IVQMNNYCGI GIDAELSLDF HQAREEEPGK FTSRFHNKGV YVRVGLQKIS HSRSLHKEIR LQVEQQEVEL PSIEGLIFIN IPSWGSGADL WGSDNDSRFE KPRIDDGLLE VVGVTGVVHM GQVQGGLRSG IRIAQGSYFR VTLLKATPVQ VDGEPWVQAP GHMIISATAP KVHMLRKAKQ KPRKAGANRD TRVDTLPAPE GNPL Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Diacylglycerol Kinase, theta 110kDa (DGKQ) (AA 1-942) protein (His tag)
custom-made DGKQ Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- DGKQ (Diacylglycerol Kinase, theta 110kDa (DGKQ))
- Alternative Name
- Dgkq (DGKQ Products)
- Background
- Diacylglycerol kinase theta (DAG kinase theta) (EC 2.7.1.107) (EC 2.7.1.93) (Diglyceride kinase theta) (DGK-theta),FUNCTION: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:26748701). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:26748701). Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription (By similarity). Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (By similarity). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis (PubMed:26748701). {ECO:0000250|UniProtKB:D3ZEY4, ECO:0000250|UniProtKB:P52824, ECO:0000269|PubMed:26748701}.
- Molecular Weight
- 102.3 kDa
- UniProt
- Q6P5E8
- AlphaFold
- Q6P5E8
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