TOPBP1 Protein (AA 1-1515) (His tag)

Catalog No. ABIN7564170

Product Details

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1515

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TOPBP1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Topbp1 Protein expressed in mammalian cells.

Sequence: MSRNDQEPFL VKFLKSSDNS ECFFKALESI KELQSEDYLQ IITDEEALKI RENDKSLYIC DRFSGTVFDH LKQLGCRIVG PQVVTFCMRH QQCVPRAEHP VYNMIMSDVT VSCTSLDKDK REEVHKYVQM MGGRVYRDLN VSVTHLIAGE VGSKKYLVAA NLKKPILLPS WIKTLWEKSQ EKKITKYTDV NMEDFKCPIF LGCIICVTGL NGIHRKTVQQ LTAKHGGQYM GQLKMNECTH LIVQEPKGQK YECARRWNVH CVTLQWFHDS IEKGFCQDES IYKAETRVEA KMVPDTSTPT AQSNAESHTL ADVSHISNIN GSCVNETMFG STTSKLECSL ENLENLDISM FQAPEDLLDG CRIYLCGFSG RKLDKLRRLI NSGGGVRFNQ LNEDVTHVIV GDYDDDVRQF WSKSSHRPHV VGAKWLLECF TKGYILPEES YIHTNYQPAG IAVSDQPGNQ TAVLDKSGSF SKSALVPAER LQQADEDLLA QYGNDDSTMV EAKLSEALEP EVGPCPGSAH REPCDDSTHI SVQEENKSSV SHCILDDSTV REEGLFSQKS FLVLGFSVEN KCNIVDIIRE HAGKIVSLPS RIVADYAVVP LLGCEVDVTV GEVVTNTWLV TCIDNQTLVD PKSNPLFTPV SVMSGVTPLE DCVISFSQCV GAERDSLVFL ANHLGASVQE FFVRKANAKK GMLASTHLIV KEPTGSKYEA AKKWSLPAVN ISWLLETARI GKRADENHFL VDNAPKQEQV LETKIPNGVS SNPDLPAHPD AHLEIHRKKA VTPLDMNRFQ SRAFRAVISQ QRGQDPTFPP VRQPLTKEPS LHLDTPSKFL SKDKLFKPSF DVTDALAALE TPNAASQKRK LSSPLSEVIV RNLTVALANS SRNTDSHSAS PQLKGAHLEE EETRKPLDSV VVCVSKKLSK KQSELNGVAA SLGAEYRWSF DETVTHFIYQ GRANDSNREY KSAKERGVHI VSEHWLLECA QEYKHLPESL YPHTYNPKMS LDINTVQDGR LCNSRAPLAV SASKDDGPDH LSVEGNETNT MGTNDKESPL LNGSGRDDCK GALTQALEMR ENFQKQLQEI MSATCIVKTP AQKTCMSRSS CNSASSTPDS ARSVRSGRSR VLEALRQSRQ AVPDVNTEPS QNEQIIWDDP TAREERARLA SNLQWPSDPT QHSELQVEIK MPDDSPSRKP VYHSEIAEQA SCVTQAPGHP GSEEPEPPVA ERPLIPEPQA PAVASPLAKP PVAPQPADKI ETQEETHRKV KKQYVFQMSS LNSQERIDYC RLIKDLGGSV IEKQCSDPSC THMVVGYPLR NEKYLASMAA GKWVLHRSYL DACKTAGRFV QEEDYEWGSS SILDALPDVT EHQQKLALAA MRWRKRIQQS QESGIVEGAF SGWKAILRVD RPREAGFKRL LQAGGAKVLS GHPEPLLKDA THLFCDFNKL KPDDCRVFIA EATAQNMVCL KTEYIADYLM LESPPCADNY RVSEAALFHN KKGGPGLPQK RKTPAENVVK RPRVH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Alternative Name: Topbp1 (TOPBP1 Products)

Background: DNA topoisomerase 2-binding protein 1 (DNA topoisomerase II-beta-binding protein 1) (TopBP1) (DNA topoisomerase II-binding protein 1),FUNCTION: Scaffold protein that acts as a key protein-protein adapter in DNA replication and DNA repair. Composed of multiple BRCT domains, which specifically recognize and bind phosphorylated proteins, bringing proteins together into functional combinations (PubMed:14718568). Required for DNA replication initiation but not for the formation of pre-replicative complexes or the elongation stages (By similarity). Necessary for the loading of replication factors onto chromatin, including GMNC, CDC45, DNA polymerases and components of the GINS complex (By similarity). Plays a central role in DNA repair by bridging proteins and promoting recruitment of proteins to DNA damage sites (By similarity). Involved in double-strand break (DSB) repair via homologous recombination in S-phase by promoting the exchange between the DNA replication factor A (RPA) complex and RAD51 (By similarity). Mechanistically, TOPBP1 is recruited to DNA damage sites in S-phase via interaction with phosphorylated HTATSF1, and promotes the loading of RAD51, thereby facilitating RAD51 nucleofilaments formation and RPA displacement, followed by homologous recombination (By similarity). Involved in microhomology-mediated end-joining (MMEJ) DNA repair by promoting recruitment of polymerase theta (POLQ) to DNA damage sites during mitosis (By similarity). MMEJ is an alternative non-homologous end-joining (NHEJ) machinery that takes place during mitosis to repair DSBs in DNA that originate in S-phase (By similarity). Recognizes and binds POLQ phosphorylated by PLK1, enabling its recruitment to DSBs for subsequent repair (By similarity). Involved in G1 DNA damage checkpoint by acting as a molecular adapter that couples TP53BP1 and the 9-1-1 complex (By similarity). In response to DNA damage, triggers the recruitment of checkpoint signaling proteins on chromatin, which activate the CHEK1 signaling pathway and block S-phase progression (By similarity). Acts as an activator of the kinase activity of ATR (By similarity). Also required for chromosomal stability when DSBs occur during mitosis by forming filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis (By similarity). Together with CIP2A, plays an essential role in the response to genome instability generated by the presence of acentric chromosome fragments derived from shattered chromosomes within micronuclei (By similarity). Micronuclei, which are frequently found in cancer cells, consist of chromatin surrounded by their own nuclear membrane: following breakdown of the micronuclear envelope, a process associated with chromothripsis, the CIP2A-TOPBP1 complex tethers chromosome fragments during mitosis to ensure clustered segregation of the fragments to a single daughter cell nucleus, facilitating re-ligation with limited chromosome scattering and loss (By similarity). Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters, thereby down-regulating E2F1 activity and inhibiting E2F1-dependent apoptosis during G1/S transition and after DNA damage (By similarity). {ECO:0000250|UniProtKB:Q800K6, ECO:0000250|UniProtKB:Q92547, ECO:0000269|PubMed:14718568}.

Molecular Weight: 168.9 kDa

UniProt: Q6ZQF0

AlphaFold: Q6ZQF0

Pathways: Chromatin Binding