MED23 Protein (AA 1-1367) (His tag)

Catalog No. ABIN7564301

Product Details

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Protein Type: Recombinant

Protein Characteristics: AA 1-1367

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This MED23 protein is labelled with His tag.

Purpose: Made-to-order recombinant Med23 Protein expressed in mammalian cells.

Sequence: METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVETG LLPPRMVCES LINSDSLEWE RTQLWALTFK LVRKIIGGVD YKGVRDLLKA ILEKILTIPN TVSSAVVQQL LAAREVIAYI LERNACLLPA YFAVTEIRKL YPEGKLPHWL LGNLVSDFVD TFRPTARINS ICGRCSLLPV VNNSGAICNS WKLDPATLRF PLKGLLPYDK DLFEPQTALL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL EDQLVDLVVY AMERSETEEK FDDGGTSQLL WQHLSSQLIF FVLFQFASFP HMVLSLHQKL AGRGLIKGRD HLMWVLLQFI SGSIQKNALA DFLPVMKLFD LLYPEKECIP VPDINKPQST HAFAMTCIWI HLNRKAQNGD STLQIPIPHS LKLHHEFLQQ SLRNKSLQMN DYKIALLCNA YSTNSECFTL PMGALVETIY GNGIMRVPLP GTSCLASASV TPLPMNLLDS LTVHAKMSLI HSIATRVIKL AHTKSSVALA PALVETYSRL LVYMEIESLG IKGFISQLLP TVFKSHAWGI LHTLLEMFSH RMHHIQPHYR VQLLSHLHTL AAVAQTNQNQ LHLCVESTAL RLITALGSSE VQPQFTRFLN DPKTVLSAES EELNRALILT LARATHVTDF FTGSDSIQGT WCKDILQTIM NFTPHNWASH TLSCFPAPLQ AFFKQNNVPQ ESRFNLKKNV EEEYRKWKSM TDENEIITQF SVQGFPPLFL CLLWKMLLET DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV SDFVKENSPE HWLQSDWHTK HMSYHKKYPE KLYFEGLAEQ VDPPVPIQSP YLPIYFGNVC LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR PVTYLYNTLH YYEMCLRNRD HLKRKLVHAI IGSLKDNRPQ GWCLSDTYLK HAMNAREDNP WVPEDSYYCK LIGRLVDTMA GKSPGPFPNC DWRFNEFPNP AAHALHVTCV ELMALAVPGK DVGNALLNVV LKSQPLVPRE NITAWMNAIG LIITALPEPY WIVLHDRIVN VISSSSLTSE TEWVGYPFRL FDFTACHQSY SEMSCSYTLA LAHAVWHHSS IGQLSLIPKF LTEALLPVVK TEFQLLYVYH LVGPFLQRFQ QERTRCMIEI GVAFYDMLLN VDQCSTHLNY MDPICDFLYH MKYMFTGDSV KEQVEKIICN LKPALKLRLR FITHISKMEP AVPPQALNSG SPAPQSNQVP ASLPVTQ Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Alternative Name: Med23 (MED23 Products)

Background: Mediator of RNA polymerase II transcription subunit 23 (Cofactor required for Sp1 transcriptional activation subunit 3) (CRSP complex subunit 3) (Mediator complex subunit 23) (Protein sur-2 homolog) (mSur-2),FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity). Also required for transcriptional activation subsequent to the assembly of the pre-initiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling. {ECO:0000250, ECO:0000269|PubMed:11934987, ECO:0000269|PubMed:14759369, ECO:0000269|PubMed:15542641, ECO:0000269|PubMed:15749018}.

Molecular Weight: 156.1 kDa

UniProt: Q80YQ2

AlphaFold: Q80YQ2

Pathways: Regulation of Lipid Metabolism by PPARalpha