CHFR Protein (AA 1-664) (His tag)
-
- Target See all CHFR Proteins
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-664
-
Origin
- Mouse
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CHFR protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Chfr Protein expressed in mammalian cells.
- Sequence
- MELHGEEQPP PPQEPWGRLL RLGAEEDEPQ ILLWKREWTI GRRRGCDLSF PSNKLVSGDH CKLTVDEISG EVTLEDTSTN GTVINKLQVV KKQTYPLQSG DIIYLVYRKN EPEHNVAYLY ESLSGKQSLT QDSLEANKEN MFHVTKDCSG PGQGDDPQVP LLSPMAQTCL EEPQPSTSTS DLLPTASTSS TEPELTSAGQ KHSSSSGPGN TSISPKGRSS LVANGELSSL SPVFQDKEAS FSLLESKDHE ELEPAKKKMK GDGELDTNLQ LLVSGQRGNA QTSSEDVKDA SVKPDKMEET LTCIICQDLL HDCVSLQPCM HTFCAACYSG WMERSSLCPT CRCPVERICK NHILNNLVEA YLIQHPDKSR SEEDVRSMDA RNKITQDMLQ PKVRRSFSDE EGSSEDLLEL SDVDSESSDI SQPYIVCRQC PEYRRQAVQS LPCPVPESEL GATLALGGEA PSTSASLPTA APDYMCPLQG SHAICTCCFQ PMPDRRAERE QDPRVAPQQC AVCLQPFCHL YWGCTRTGCF GCLAPFCELN LGDKCLDGVL NNNNYESDIL KNYLATRGLT WKSVLTESLL ALQRGVFMLS DYRITGNTVL CYCCGLRSFR ELTYQYRQNI PASELPVTVT SRPDCYWGRN CRTQVKAHHA MKFNHICEQT RFKN Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
-
Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
-
Want other Options for this Protein ?
!Discover Our Catalog and Custom Protein Service Options!Your project requires further customization? Contact us and discover our custom protein solutions
-
Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 1-664) protein (His tag)
custom-made CHFR Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 1-652) protein (GST tag)CHFR Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Checkpoint with Forkhead and Ring Finger Domains (CHFR) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CHFR Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
-
- Target
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
- Alternative Name
- Chfr (CHFR Products)
- Background
- E3 ubiquitin-protein ligase CHFR (EC 2.3.2.27) (Checkpoint with forkhead and RING finger domains protein) (RING-type E3 ubiquitin transferase CHFR),FUNCTION: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress (By similarity). {ECO:0000250|UniProtKB:Q96EP1, ECO:0000269|PubMed:15793587}.
- Molecular Weight
- 73.9 kDa
- UniProt
- Q810L3
- AlphaFold
- Q810L3
-
