MGAT4B Protein (AA 1-548) (His tag)

Catalog No. ABIN7564323

Product Details

Target: MGAT4B (Mannosyl (Alpha-1,3-)-Glycoprotein beta-1,4-N-Acetylglucosaminyltransferase, Isozyme B (MGAT4B))

Protein Type: Recombinant

Protein Characteristics: AA 1-548

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This MGAT4B protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Mgat4b Protein expressed in mammalian cells.

Sequence: MRLRNGTFLT LLLFCLCAFL SLSWYAALSG QKGDVVDIYQ REFLALRDRL HAAEQESLKR SKELNLVLEE IKRAVSERQA LRDGEGNRTW GRLTEDPRLK PWNVSHRHVL HLPTVFHHLP HLLAKESSLQ PAVRVGQGRT GVSVVMGIPS VRREVHSYLT DTLHSLISEL SPQEKEDSVI VVLIAETDPQ YTSAVTENIK ALFPTEIHSG LLEVISPSPH FYPDFSRLRE SFGDPKERVR WRTKQNLDYC FLMMYAQSKG IYYVQLEDDI VAKPNYLSTM KNFALQQPSE DWMILEFSQL GFIGKMFKSL DLSLIVEFIL MFYRDKPIDW LLDHILWVKV CNPEKDAKHC DRQKANLRIR FKPSLFQHVG THSSLAGKIQ KLKDKDFGKH ALRKEHVNPP AEVSTSLKTY QHFTLEKAYL REDFFWAFTP AAGDFIRFRF FQPLRLERFF FRSGNIEHPE DKLFNTSVEV LPFDNPQSEK EALQEGRSAT LRYPRSPDGY LQIGSFYKGV AEGEVDPAFG PLEALRLSIQ TDSPVWVILS EIFLKKAD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MGAT4B (Mannosyl (Alpha-1,3-)-Glycoprotein beta-1,4-N-Acetylglucosaminyltransferase, Isozyme B (MGAT4B))

Alternative Name: Mgat4b (MGAT4B Products)

Background: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B (EC 2.4.1.145) (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb) (GlcNAc-T IVb) (GnT-IVb) (N-acetylglucosaminyltransferase IVb) (UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb),FUNCTION: Glycosyltransferase that catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains. Prefers complex-type N-glycans over hybrid-types. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis. {ECO:0000250|UniProtKB:Q9UQ53}.

Molecular Weight: 63.3 kDa

UniProt: Q812F8

AlphaFold: Q812F8