FBXL2 Protein (AA 1-423) (His tag)
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- Target See all FBXL2 Proteins
- FBXL2 (F-Box and Leucine-Rich Repeat Protein 2 (FBXL2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-423
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FBXL2 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Fbxl2 Protein expressed in mammalian cells.
- Sequence
- MVFSNSDDGL INKKLPKELL LRIFSFLDIV TLCRCAQISK AWNILALDGS NWQRVDLFNF QTDVEGRVVE NISKRCGGFL RKLSLRGCIG VGDSSLKTFA QNCRNIEHLN LNGCTKITDS TCYSLSRFCS KLKHLDLTSC VSVTNSSLKG ISEGCRNLEY LNLSWCDQIT KEGIEALVRG CRGLKALLLR GCTQLEDEAL KHIQNHCHEL VSLNLQSCSR ITDDGVVQIC RGCHRLQALC LSGCSNLTDA SLTALGLNCP RLQVLEAARC SHLTDAGFTL LARNCHELEK MDLEECVLIT DSTLVQLSIH CPKLQALSLS HCELITDEGI LHLSSSTCGH ERLRVLELDN CLLVTDASLE HLENCRGLER LELYDCQQVT RAGIKRMRAQ LPHVKVHAYF APVTPPPAVA GSGHRLCRCC VIL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
- Top Product
- Discover our top product FBXL2 Protein
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F-Box and Leucine-Rich Repeat Protein 2 (FBXL2) (AA 1-423) protein (His tag)
custom-made FBXL2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
F-Box and Leucine-Rich Repeat Protein 2 (FBXL2) (AA 1-423) protein (GST tag)FBXL2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
F-Box and Leucine-Rich Repeat Protein 2 (FBXL2) (AA 1-423), (full length) protein (His-SUMO Tag)FBXL2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
F-Box and Leucine-Rich Repeat Protein 2 (FBXL2) protein (Myc-DYKDDDDK Tag)FBXL2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- FBXL2 (F-Box and Leucine-Rich Repeat Protein 2 (FBXL2))
- Alternative Name
- Fbxl2 (FBXL2 Products)
- Background
- F-box/LRR-repeat protein 2 (F-box and leucine-rich repeat protein 2),FUNCTION: Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21343341, PubMed:23542741). Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin (By similarity). This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin (By similarity). Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0) (By similarity). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (By similarity). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (PubMed:21343341). The SCF(FBXL2) complex acts as a regulator of inflammation by mediating ubiquitination and degradation of TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF4, TRAF5 and TRAF6) (PubMed:23542741). The SCF(FBXL2) complex acts as a negative regulator of the NLRP3 inflammasome by mediating ubiquitination and degradation of NLRP3 (By similarity). {ECO:0000250|UniProtKB:Q9UKC9, ECO:0000269|PubMed:21343341, ECO:0000269|PubMed:23542741}.
- Molecular Weight
- 46.9 kDa
- UniProt
- Q8BH16
- AlphaFold
- Q8BH16
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