Casein Kinase 1 gamma 2 Protein (AA 1-415) (His tag)
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- Target See all Casein Kinase 1 gamma 2 (CSNK1G2) Proteins
- Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-415
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Casein Kinase 1 gamma 2 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Csnk1g2 Protein expressed in mammalian cells.
- Sequence
- MDFDKKGGKG ELEEGRRMSK TGTSRSNHGV RSSGTSSGVL MVGPNFRVGK KIGCGNFGEL RLGKNLYTNE YVAIKLEPIK SRAPQLHLEY RFYKQLSTTE GVPQVYYFGP CGKYNAMVLE LLGPSLEDLF DLCDRTFTLK TVLMIAIQLI TRMEYVHTKS LIYRDVKPEN FLVGRPGSKR QHSIHIIDFG LAKEYIDPET KKHIPYREHK SLTGTARYMS INTHLGKEQS RRDDLEALGH MFMYFLRGSL PWQGLKADTL KERYQKIGDT KRATPIEVLC ESFPEEMATY LRYVRRLDFF EKPDYDYLRK LFTDLFDRSG YVFDYEYDWA GKPLPTPIGT VHPDVPSQPP HRDKAQLHTK NQALNSTNGE LNTDDPTAGH SNAPIAAPAE VEVADETKCC CFFKRRKRKS LQRHK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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Casein Kinase 1, gamma 2 (CSNK1G2) (AA 1-415) protein (His tag)
custom-made CSNK1G2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Casein Kinase 1, gamma 2 (CSNK1G2) (AA 1-415) protein (GST tag)CSNK1G2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Casein Kinase 1, gamma 2 (CSNK1G2) (AA 18-415) protein (His tag)CSNK1G2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Casein Kinase 1, gamma 2 (CSNK1G2) (Active) protein (His tag)CSNK1G2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 80 % as determined by reducing SDS-PAGE. Active
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))
- Alternative Name
- Csnk1g2 (CSNK1G2 Products)
- Background
- Casein kinase I isoform gamma-2 (CKI-gamma 2) (EC 2.7.11.1),FUNCTION: Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates MTA1 (PubMed:15077195). Phosphorylates COL4A3BP/CERT and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (By similarity). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (PubMed:16014721). Involved in regulation of reactive oxygen species (ROS) levels (By similarity). {ECO:0000250|UniProtKB:P48729, ECO:0000250|UniProtKB:P78368, ECO:0000269|PubMed:15077195, ECO:0000269|PubMed:16014721}.
- Molecular Weight
- 47.6 kDa
- UniProt
- Q8BVP5
- AlphaFold
- Q8BVP5
- Pathways
- Hedgehog Signaling
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