E4F1 Protein (AA 1-783) (His tag)

Catalog No. ABIN7564534

This Recombinant E4F1 protein is produced in HEK-293 Cells.

Quick Overview for E4F1 Protein (AA 1-783) (His tag) (ABIN7564534)

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-783

Purification tag / Conjugate: This E4F1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant E4f1 Protein expressed in mammalian cells.

Sequence: MEGAMAVRVT AAHTAEARAE AGREAGEGGV AAAAALSSGG FLGLPAPFSE EDEDDVHRCG RCQVEFTALE DFVQHKIQKT CHRAPQEALP TTPAATALLD QEVVPTAAEG GPDEPITVAH IVVEATSLAE DISHAPDLVG SGHIKEVIVA AEAEPGDVEM AEAPGSPNHQ ELGLLGEGEQ AHVKLLVNKE GRYVCMLCHK TFKTGSILKA HMVTHSSRKD HECKLCGASF RTKGSLIRHH RRHTDERPYK CAKCGKSFRE SGALTRHLKS LTPCTEKIRF SISKDTAVGK EEVPAGSSAS TVGTVTSSVA GDPMETSPVI HLVTDAKGTV IHEVHVQMQE LPLGMKALTP ESPDSEELPC SSENSRENLL HQAMQNSGIV LERVAGEESA LEPAPPSGSS PQCLGDGSPE LPLLKVEQIE TQVASEAATV PRTHPCPQCS ETFPTAATLE AHKRGHIAPR PFTCTQCGKA FPKAYLLKKH QEVHVHERRF RCGDCGKLYK TIAHVRGHRR VHSDERPFPC PQCGKRYKTK NAQQVHFRTH LEEKPHVCQF CSRGFREKGS LVRHVRHHTG EKPFKCYKCG RGFAEHGTLN RHLRTKGGCL LEVEELLVSE ESPSAAATVL AEDPHTVLVE FSSVVADTQE YIIEATADDT ETSEATEIIE GTQTEVDSHI MKVVQQIVHQ AGAGHQIIVQ NVTMDQETAL GSEATAADTI TIATPESLTE QVAMTLASAI SEGTVLTARA GPNSTEQATV TMVSSEDIEI LEHGGELVIA SPEGQLEVQT VIV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Alternative Name: E4f1

Background: Transcription factor E4F1 (EC 2.3.2.27) (E4F transcription factor 1) (Putative E3 ubiquitin-protein ligase E4F1) (RING-type E3 ubiquitin transferase E4F1) (Transcription factor E4F) (Transcription factor phi AP3) (p120E4F),FUNCTION: May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription. {ECO:0000269|PubMed:10644996, ECO:0000269|PubMed:15226446, ECO:0000269|PubMed:17557114, ECO:0000269|PubMed:8262041}.

Molecular Weight: 84.3 kDa

UniProt: Q8CCE9

AlphaFold: Q8CCE9