PASK Protein (AA 1-1383) (His tag)

Catalog No. ABIN7564552

This Recombinant PASK protein is produced in HEK-293 Cells.

Quick Overview for PASK Protein (AA 1-1383) (His tag) (ABIN7564552)

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1383

Purification tag / Conjugate: This PASK protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pask Protein expressed in mammalian cells.

Sequence: MEDRGPPVFA EDWKCLSESP PVQEGPAAQA TFEPSKPLSI AHKHLSRKNG LSRLCQSRMA LSEDRWSSYC LSSLAAQNIC TSKLHCAAAP EYADPTAGPL GSTSCCSLLR GLASGCSGSL LSTPVCNPNK AVFTVDAKTT EILVANDKAC SLLGYSSHDL IGQKLAQFFL KSDSEVVEAL SEEHVEADGH AAVVFGTVVD IVSRIGEKIP VSVWIKRLQQ DRGLCCVVVL EPVERVSAWV AFQSDGTITS CDSLFAHLHG FTSPKDVVGQ CVIDLIPSMQ LPPPGQHIPK SLKIQRSVGR ARDGTTFPLS LKLKSKPSGR AVADSEAASE PGYQASVWVF CTISGLITLL PDGTIYGVNH SFALMLFGYG KTELLGKNIT FLIPGFYHYM DLTYDSSVQL PDLVNCLDIG RKSGPGEMNS DAQHNWELAS GAQGPRIDVV LARDHMPSQD ETLKLVGGQV SSRTQTRLET GYKILPSSAC QPSLGVDSNP EDGEQSLLTD QQSIPKRNLP AHGGQNQLDT SEISLPVLKE HLLSEIQKNI SEESPLTHRK WLSKVQQNPT KGSLPIHEEQ LLFAGQHIHV LGKEDPSAAE SYRESLLEES KSKPVDAKLF ASCEDSEPLV SVKDRGSSVD TCNLHQEAQL ELMGVSSPNP WADATMPEPH TTGQIAGGSL TYCPQYRSEW ASQQRGQDSA PSPSGMACVL LGTPTLDEPW PGVRNDREEL QTCLIKEQLS KSSCEGNLGI SRVELVPEEH PPFTAPVSFC DLGGRDLHAS RSGSSSACYA LATDLPGVLE AVEAQEADVN SYSWNLKELF LKDQTDRTPS HCSCTTSELS EAPSLSVVGS DLDVGILHRQ TSDILVDREM LLLTGTYFDL SEGQRFQEMG AGHDRAELSN ISLVSSEHYE TSDIESPGCD PPLPDPGPND MCLSAEKPRP SAQITSTPVA RGATSLQQEI QEGIYSGSCY HRDGLQLSIQ FEVKRVELQG SATLFCCWLV KDLFHSHRDS ATRTRLFLAS LPSSTHSMPE LSGSSFGEVL RAKPWFEESP TPAELEGLAA CEGEYDYKYN TISPLGSGAF GFVWTAVEKE CNKEVVVKFI KKEKVLEDCW IEDPKLGRVT LEIAILSKVD HANIIKVLDI FENQEFFQLV MEKHGSGMDL FAFIDHHPCL DEPLASFIFR QLVSAVGYLH SQGIIHRDIK DENIVIAEDF TIKLIDFGSA AYLERGKLFY TFCGTIEYCA PEVLIGNPYR GPELEMWSLG VTLYTLIFEE NPFCEVEETM EAVIHPPFLV SQELMSLLSG LLQPCPEQRT TLEKLIRDPW VTQPVNLASY TWEEVCRTNQ PESGLLSAAS LEIGSRSPSE MAQREGLCGP PAPRETRGDQ HCLHLKDPSL PVS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Alternative Name: Pask

Background: PAS domain-containing serine/threonine-protein kinase (PAS-kinase) (PASKIN) (EC 2.7.11.1),FUNCTION: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells, however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation. {ECO:0000269|PubMed:15148392, ECO:0000269|PubMed:17878307, ECO:0000269|PubMed:18509100, ECO:0000269|PubMed:21181396}.

Molecular Weight: 151.3 kDa

UniProt: Q8CEE6

AlphaFold: Q8CEE6

Pathways: Regulation of Carbohydrate Metabolic Process