PDE5A Protein (AA 1-865) (His tag)

Catalog No. ABIN7564563

Product Details

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Protein Type: Recombinant

Protein Characteristics: AA 1-865

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This PDE5A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pde5a Protein expressed in mammalian cells.

Sequence: MERAGPNSVR SQQQRDPDWV EAWLDDHRDF TFSYFIRKAT RDMVNAWFSE RVHNIPVCKE GIRAHTESCS CSLQQSPHAD NTTPGAPARK ISASEFDRPL RPIVVKDSEG TVSFLSDSGK KEQMPLTPPR FDSDEGDQCS RLLELVKDIS SHLDVTALCH KIFLHIHGLI SADRYSLFLV CEDSSKDKFL ISRLFDVAEG STLEEASNNC IRLEWNKGIV GHVAAFGEPL NIKDAYEDPR FNAEVDQITG YKTQSILCMP IKNHREEVVG VAQAINKKSG NGGTFTEKDE KDFAAYLAFC GIVLHNAQLY ETSLLENKRN QVLLDLASLI FEEQQSLEVI LKKIAATIIS FMQVQKCTIF IVDEDCPDSF SRVFHMECEE VGKPSDPLTR EQDANKINYM YAQYVKNTME PLNIPDVTKD KRFPWTNENM GHVNTPCIGS LLCTPIKNGK KNKVIGVCQL VNKMEENTGK IKAFNQNDEQ FLEAFVIFCG LGIQNTQMYE AVERAMAKQM VTLEVLSYHA SAAEEETREL QALSAAVVPS AQTLKITDFS FSDFELSDLE TALCTIRMFT DLNLVQNFQM KHEVLCRWIL SVKKNYRKNV AYHNWRHAFN TAQCMFAALK AGKIQNKLTD LETLALLIAA LSHDLDHRGV NNSYIQRSEH PLAQLYCHSI MEHHHFDQCL MILNSPGNQI LSGLSIDEYK TTLKIIKQAI LATDLALYIK RRGEFFELIR KNQFSFEDPL QKELFLAMLM TACDLSAITK PWPIQQRIAE LVAAEFFDQG DRERKELNME PADLMNREKK NKIPSMQVGF IDAICLQLYE ALTHVSEDCL PLLDGCRKNR QKWQALAEQQ EKMLLNGESS QGKRD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Alternative Name: Pde5a (PDE5A Products)

Background: CGMP-specific 3',5'-cyclic phosphodiesterase (EC 3.1.4.35) (cGMP-binding cGMP-specific phosphodiesterase) (CGB-PDE),FUNCTION: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP. {ECO:0000250|UniProtKB:O76074}.

Molecular Weight: 98.4 kDa

UniProt: Q8CG03

AlphaFold: Q8CG03

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling