AIM2 Protein (AA 1-354) (His tag)

Catalog No. ABIN7564785

Product Details

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Protein Type: Recombinant

Protein Characteristics: AA 1-354

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This AIM2 protein is labelled with His tag.

Purpose: Made-to-order recombinant Aim2 Protein expressed in mammalian cells.

Sequence: MESEYREMLL LTGLDHITEE ELKRFKYFAL TEFQIARSTL DVADRTELAD HLIQSAGAAS AVTKAINIFQ KLNYMHIANA LEEKKKEAER KLMTNTKKRG TQKVENRSQA ENCSAASATR SDNDFKEQAA TEVCPQAKPQ KKQMVAEQEA IREDLQKDPL VVTVLKAINP FECETQEGRQ EIFHATVATE TDFFFVKVLN AQFKDKFIPK RTIKISNYLW HSNFMEVTSS SVVVDVESNH EVPNNVVKRA RETPRISKLK IQPCGTIVNG LFKVQKITEE KDRVLYGIHD KTGTMEVLVL GNPSKTKCEE GDKIRLTFFE VSKNGVKIQL KSGPCSFFKV IKAAKPKTDM KSVE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Alternative Name: Aim2 (AIM2 Products)

Background: Interferon-inducible protein AIM2 (Interferon-inducible protein 210) (Ifi-210) (Interferon-inducible protein p210),FUNCTION: Sensor component of the AIM2 inflammasome, which mediates inflammasome activation in response to the presence of double-stranded DNA (dsDNA) in the cytosol, leading to subsequent pyroptosis (PubMed:19158679, PubMed:19131592, PubMed:20351692, PubMed:19158675, PubMed:19158676, PubMed:20351693, PubMed:20417169, PubMed:20457908, PubMed:21902795, PubMed:32350463, PubMed:34006824, PubMed:23567559). Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation (PubMed:19131592, PubMed:20351692, PubMed:19158675, PubMed:19158676, PubMed:20351693, PubMed:20417169, PubMed:20457908, PubMed:21902795, PubMed:23567559, PubMed:32350463). Acts as a recognition receptor (PRR): specifically recognizes and binds dsDNA in the cytosol, and mediates the formation of the inflammasome polymeric complex composed of AIM2, CASP1 and PYCARD/ASC (PubMed:19131592, PubMed:20351692, PubMed:19158675, PubMed:19158676, PubMed:20351693, PubMed:20417169, PubMed:20457908, PubMed:21902795, PubMed:23567559). Recruitment of pro-caspase-1 (proCASP1) to the AIM2 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion (PubMed:19158676). In some cells, CASP1 activation mediates cleavage and activation of GSDMD, triggering pyroptosis without promoting cytokine secretion (PubMed:27846608, PubMed:32350463). Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner (PubMed:19131592, PubMed:19158675, PubMed:20351692, PubMed:23567559). Involved in the DNA damage response caused by acute ionizing radiation by mediating pyroptosis of intestinal epithelial cells and bone marrow cells in response to double-strand DNA breaks (PubMed:27846608). Mechanistically, AIM2 senses DNA damage in the nucleus to mediate inflammasome assembly and inflammatory cell death (PubMed:27846608). Also acts as a regulator of neurodevelopment via its role in the DNA damage response: acts by promoting neural cell death in response to DNA damage in the developing brain, thereby purging genetically compromised cells of the central nervous system (PubMed:32350463). Pyroptosis mediated by the AIM2 inflammasome in response to DNA damage is dependent on GSDMD without involving IL1B and IL18 cytokine secretion (PubMed:27846608, PubMed:32350463). Also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection (PubMed:34471287). Can also trigger PYCARD/ASC-dependent, caspase-1-independent cell death that involves caspase-8 (CASP8) (PubMed:22555457). {ECO:0000269|PubMed:19131592, ECO:0000269|PubMed:19158675, ECO:0000269|PubMed:19158676, ECO:0000269|PubMed:19158679, ECO:0000269|PubMed:20351692, ECO:0000269|PubMed:20351693, ECO:0000269|PubMed:20417169, ECO:0000269|PubMed:20457908, ECO:0000269|PubMed:21902795, ECO:0000269|PubMed:22555457, ECO:0000269|PubMed:23567559, ECO:0000269|PubMed:27846608, ECO:0000269|PubMed:32350463, ECO:0000269|PubMed:34006824, ECO:0000269|PubMed:34471287}., FUNCTION: Also acts as a tumor suppressor independently of its role in inflammatory response (PubMed:26095253). Able to suppress overt cell proliferation in enterocytes: restricts stem cell proliferation in the intestinal mucosa in an inflammasome-independent manner, contributing to a decrease in the likelihood of colorectal cancer development (PubMed:26095253, PubMed:26107252). AIM2 suppresses cell proliferation by inhibiting phosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (PubMed:26095253, PubMed:26107252). Inhibits AKT1 phosphorylation both by inhibiting the activity of PRKDC/DNA-PK kinase and promoting dephosphorylation by PP2A phosphatase (PubMed:26107252, PubMed:33505023). Also acts as a key regulator of regulatory T-cells (Treg) homeostasis by promoting their stability: acts by preventing AKT1 activation (PubMed:33505023). Its role in Treg homeostasis is important to restain autoimmune diseases (PubMed:33505023). {ECO:0000269|PubMed:26095253, ECO:0000269|PubMed:26107252, ECO:0000269|PubMed:33505023}.

Molecular Weight: 40.2 kDa

UniProt: Q91VJ1

AlphaFold: Q91VJ1

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome