AIM2 Protein (GST tag)

Catalog No. ABIN7317509

Product Details

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This AIM2 protein is labelled with GST tag.

Purpose: Recombinant Human AIM2 Protein (GST Tag)(Active)

Sequence: Met 1-Thr 343

Characteristics: A DNA sequence encoding the human AIM2 (NP_004824.1) (Met 1-Thr 343) was fused with the GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-40ug/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 1M NaCl, 0.5 mM PMSF, 5 mM GSH, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Alternative Name: AIM2 (AIM2 Products)

Synonyms: AIM2 Protein, PYHIN4 Protein, Gm1313 Protein, Ifi210 Protein, absent in melanoma 2 Protein, AIM2 Protein, Aim2 Protein

Background:

Background: AIM2, Absent In Melanoma 2 is a member of the interferon-inducible HIN-200 protein family that contains an amino-terminal pyrin domain and a carboxy-terminal oligonucleotide/oligosaccharide-binding domain, senses cytoplasmic DNA by means of its oligonucleotide/oligosaccharide-binding domain and interacts with ASC (apoptosis-associated speck-like protein containing a CARD) through its pyrin domain to activate caspase-1. In response to foreign cytoplasmic DNA, AIM2 forms an inflammasome, resulting in caspase activation in inflammatory cells. It had been pointed to a role of AIM2 function in both inflammation and cancer. AIM-2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM-2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients.

Synonym: PYHIN4

Molecular Weight: 65.2 kDa

NCBI Accession: NP_004824

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome