ALPK1 Protein (AA 1-1231) (His tag)

Catalog No. ABIN7564957

This Recombinant ALPK1 protein is produced in HEK-293 Cells.

Quick Overview for ALPK1 Protein (AA 1-1231) (His tag) (ABIN7564957)

Target: ALPK1 (alpha-Kinase 1 (ALPK1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1231

Purification tag / Conjugate: This ALPK1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Alpk1 Protein expressed in mammalian cells.

Sequence: MNNQDAVASI LHECKQVLDR LLLETPDVST EDKSEDQRCR ASLPSELRTL IQEAEEMKWP FVPEKWQYKQ AMSPEDKTNL QDVIGAGLQQ LLAALRASIL VQDCAAASAI VFLMDRFLYG LDVSGKLLQV AKGLHKLKPA TPIAPQVVIR QARVSVNSGK LLKAEYILSS LISNNGATGT WLYRNESDKV LVQSVCIQIR GQILQKLGMW YEAAELIWAS VIGYLTLPQP DKKGISTSLG ILADIFVSMS KTDYEKFKKS PKVNLALLKE FDHHLLSAAE ACKLAAAFSA YTPLFVLRAV NIRGTCLLSY SCSADCPPGM KSVHLCEAKE AFEIGLLTKK DGELVSGKQE LHSFIKAAFG LTTVHSRLHG ETDAVRAARQ LCSEAVGKLY TFSTSPTSQD REGLSQEIMS LISQVKGHLR VQSFPNLDVC SYVPESFKCG LDRLILHGHV DFQQILETYS QHHTSVCEVF ESTCGNSKSN QRDTKSEVCI TTLKTETNTA DTMVATLERV SSQDSRSTAS SKMSKKDQGK LQRERGRSWT RSKAFRVSLD LDMETETEPP NHSNGGTDVF NKSLRDNSSS CSWGRLSGLS SSTSWEEVNC AVQDVVRKGS GQEKHPVEAQ SSEAVSEEPK RNRSSRAVFL SSKLRGVSLQ TTGDDNLESS PSQLHNHTSI LPFNAKDTCL ASGAGLVETA EGSNNTSLQS SHSCGSDSWS LSSSDRFTDV TTNPSVQEEE PSGIMGDVPE SKYDFKDWHG EKNGGTLTEI CTGPELTFAP SSVDPEGETA ESTDDGLSPS QVALGCLEGS HSMSTRRTFF PDGSVQNADS AKTGCSVRDQ TVDPDASTVD EEGQMLDSTE VCSIGQDGAH RPRALRSGQS AEGPKSFVNG SRPSPIFDED FSTTEEGEEL GSMLKSSQNS SSYSPWWLKS PAFSRSSSDG ESSWSLLNSS RSSFASLAGQ TSQEILEART LQPDDLEKLL AGVRHDWLLQ RLENTGVLKS NQLQQAHSAL LLKYSKKSEL WTAQETVVYL GDYLKVKKKG KQRNAFWVHY LHQEETLGRY VGKEYKERKG LRHHFTDVER QMTAQHYVTE FNKRLYEQKI PTQIFYVPST ILLILEDRTI KGCISVEPYI LGEFVKLSNN TKVVKNEYKA TEYGLAYGHF SYEFSNHRDV VVDLQGWVTG NGKGLIYLTD PQIHSVDQKD VTTNFGKRGI FYFFNNQHAS CNEICHRLSL TRPSLEQTSK V Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ALPK1 (alpha-Kinase 1 (ALPK1))

Alternative Name: Alpk1

Background: Alpha-protein kinase 1 (EC 2.7.11.1),FUNCTION: Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (By similarity). Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria (PubMed:30111836). ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (By similarity). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (By similarity). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (By similarity). May play a role in ciliogenesis (By similarity). {ECO:0000250|UniProtKB:Q96QP1, ECO:0000269|PubMed:30111836}.

Molecular Weight: 136.1 kDa

UniProt: Q9CXB8

AlphaFold: Q9CXB8