RNF6 Protein (AA 1-667) (His tag)

Catalog No. ABIN7565010

Product Details

Target: RNF6 (RING Finger Protein 6 (RNF6))

Protein Type: Recombinant

Protein Characteristics: AA 1-667

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This RNF6 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rnf6 Protein expressed in mammalian cells.

Sequence: MDPSRSRSGG SGEESSFQEN ERRWQQERLH REEAYYQFIN ELSDEDYRLM RDHNLLGTPG EITSEELQQR LERAKEQLAS QPGSDSAASD GDSESLRAHS DEDSLLRWLN TFRRTGNVTR SGQNGNQSWR AVSRTNPNSG EFGFSLEIHI NPDNRGSEMH GEDSTDIPLS GVNREHRQQR PSSPVARRTR SQTSMSSSGP RGRRGARRQG SVQGSFATLG RLRNGIGVAL GVPRVSAPRT NVINSHTNQS DGSTLRQGGR QRFGAAHIWE NGARSNVTVR NTNQRLEPIR LRPAFSSRSR SPIQRQNGTV HHNSQRQGRP VQQTGRNRSV RHRGVTRVFL EQGREHRGTD YTPLSNSRLV SRITVEEGES SRSSAATQRH PAITLDLQVR RIRPGETRDR DSIANRTRSR AGLAESTVES TSGGFHRTIS HLERSGVRTY VSTITVPLRR ISENDVVEPS SVALRSILRQ IMTGFGELSS LMEVEPASEN QSNGQRLPEV YLELSNGDAA DDSGQHGRAS SQASQAQDGA EMLAVREPAP PQARPSGSRS RRQLGRADSV VEAGTLPILR LAHFFLLNEG DDDPIRGLTK EQIDNLSTRS YEQDGVDSEL GKVCSVCISD YVAGNKLRQL PCLHEFHIHC IDRWLSENCT CPVCRRPVLE FGATSSG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RNF6 (RING Finger Protein 6 (RNF6))

Alternative Name: Rnf6 (RNF6 Products)

Background: E3 ubiquitin-protein ligase RNF6 (EC 2.3.2.27) (RLIM-like protein),FUNCTION: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (PubMed:16204183). Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover (PubMed:16204183). Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity (By similarity). May also bind DNA and function as a transcriptional regulator (PubMed:11971979). Mediates polyubiquitination of QKI in macrophages, leading to its degradation (PubMed:36088389). {ECO:0000250|UniProtKB:Q9Y252, ECO:0000269|PubMed:11971979, ECO:0000269|PubMed:16204183, ECO:0000269|PubMed:36088389}.

Molecular Weight: 74.1 kDa

UniProt: Q9DBU5

AlphaFold: Q9DBU5

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Cell Size