CPSF1 Protein (AA 1-1441) (His tag)

Catalog No. ABIN7565028

Product Details

Target: CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1441

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CPSF1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Cpsf1 Protein expressed in mammalian cells.

Sequence: MYAVYKQAHP PTGLEFTMYC NFFNNSERNL VVAGTSQLYV YRLNRDAEAL TKNDGSTEGK AHREKLELVA SFSFFGNVMS MASVQLAGAK RDALLLSFKD AKLSVVEYDP GTHDLKTLSL HYFEEPELRD GFVQNVHTPR VRVDPDGRCA AMLIYGTRLV VLPFRRESLA EEHEGLMGEG QRSSFLPSYI IDVRALDEKL LNIIDLQFLH GYYEPTLLIL FEPNQTWPGR VAVRQDTCSI VAISLNITQK VHPVIWSLTS LPFDCTQALA VPKPIGGVVI FAVNSLLYLN QSVPPYGVAL NSLTTGTTAF PLRTQEGVRI TLDCAQAAFI SYDKMVISLK GGEIYVLTLI TDGMRSVRAF HFDKAAASVL TTSMVTMEPG YLFLGSRLGN SLLLKYTEKL QEPPASSVRE AADKEEPPSK KKRVEPAVGW TGGKTVPQDE VDEIEVYGSE AQSGTQLATY SFEVCDSMLN IGPCANAAVG EPAFLSEEFQ NSPEPDLEIV VCSGYGKNGA LSVLQKSIRP QVVTTFELPG CYDMWTVIAP VRKEEEETPK AESTEQEPSA PKAEEDGRRH GFLILSREDS TMILQTGQEI MELDTSGFAT QGPTVFAGNI GDNRYIVQVS PLGIRLLEGV NQLHFIPVDL GAPIVQCAVA DPYVVIMSAE GHVTMFLLKS DSYGGRHHRL ALHKPPLHHQ SKVIALCLYR DVSGMFTTES RLGGARDELG GRSGSEAEGL GSETSPTVDD EEEMLYGDSS ALFSPSKEEA RRSSQPPADR DPAPFKADPT HWCLLVRENG TMEIYQLPDW RLVFLVKNFP VGQRVLVDSS FGQPTTQGEV RKEEATRQGE LPLVKEVLLV ALGSRQSRPY LLVHVDQELL IYEAFPHDSQ LGQGNLKVRF KKVPHNINFR EKKPKPSKKK AEGCSTEEGS GGRGRVARFR YFEDIYGYSG VFICGPSPHW LLVTGRGALR LHPMGIDGPI DSFAPFHNVN CPRGFLYFNR QGELRISVLP AYLSYDAPWP VRKIPLRCTA HYVAYHVESK VYAVATSTNT PCTRIPRMTG EEKEFEAIER DDRYIHPQQE AFSIQLISPV SWEAIPNARI ELEEWEHVTC MKTVSLRSEE TVSGLKGYVA AGTCLMQGEE VTCRGRILIM DVIEVVPEPG QPLTKNKFKV LYEKEQKGPV TALCHCNGHL VSAIGQKIFL WSLRASELTG MAFIDTQLYI HQMISVKNFI LAADVMKSIS LLRYQEESKT LSLVSRDAKP LEVYSVDFMV DNAQLGFLVS DRDRNLMVYM YLPEAKESFG GMRLLRRADF HVGAHVNTFW RTPCRGAAEG PSKKSVVWEN KHITWFATLD GGIGLLLPMQ EKTYRRLLML QNALTTMLPH HAGLNPRAFR MLHVDRRILQ NAVRNVLDGE LLNRYLYLST MERSELAKKI GTTPDIILDD LLETDRVTAH F Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))

Alternative Name: Cpsf1 (CPSF1 Products)

Background: Cleavage and polyadenylation specificity factor subunit 1 (Cleavage and polyadenylation specificity factor 160 kDa subunit) (CPSF 160 kDa subunit),FUNCTION: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction (By similarity). May play a role in eye morphogenesis and the development of retinal ganglion cell projections to the midbrain (By similarity). {ECO:0000250|UniProtKB:A0A0R4IC37, ECO:0000250|UniProtKB:Q10570}.

Molecular Weight: 160.8 kDa

UniProt: Q9EPU4

AlphaFold: Q9EPU4