PIK3AP1 Protein (AA 1-811) (His tag)

Catalog No. ABIN7565032

This Recombinant PIK3AP1 protein is produced in HEK-293 Cells.

Quick Overview for PIK3AP1 Protein (AA 1-811) (His tag) (ABIN7565032)

Target: PIK3AP1 (phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-811

Purification tag / Conjugate: This PIK3AP1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pik3ap1 Protein expressed in mammalian cells.

Sequence: MAASGWGRGC DILIFYSPDA EEWCQYLQDL FVSCRQVRSQ KTQTYRLVPD ASFSAQDLWV FRDARCVLVL LSAGLVGCFG QPGLLPMLQR ACHPPQRVVR LLCGVQPGDE DFQAFFPDWA HWQEMTCDDE PETYLAAVRK AISEDSGCDS VTDTEPEDER ELPFSKQTNL PPEISPGNLM VVQPDRIRCG AETTVYIIVR CKLDEKVSTE AEFSPEDSPS IRVEGTLENE YTVSVKAPDL SSGNVSLKVY SGDLVVCETT VSYYTDMEEI GNLLSSAANP VEFMCQAFKI VPYNTETLDK LLTESLKNNI PASGLHLFGI NQLEEDDMMT NQRDEELPTL LHFAAKYGLK NLTALLLTCP GALQAYSVAN KHGHYPNTIA EKHGFRDLRQ FIDEYVETVD MLKTHIKEEL MQGEEADDVY ESMAHLSTDL LMKCSLNPGC DDELYESMAA FAPAATEDLY VEMLQASAGN PVSGESFSRP TKDSMIRKFL EGNSVKPASW EREQHHPYGE ELYHIVDEDE TFSVDLANRP PVPVPRPEAS APGPPPPPDN EPYISKVFAE KSQERLGNFY VSSESIRKEP LVRPWRDRPP SSIYDPFAGM KTPGQRQLIT LQEQVKLGIV NVDEAVLHFK EWQLNQKKRS ESFRFQQENL KRLRESITRR RKEKPKSGKH TDLEITVPIR HSQHLPEKVE FGVYESGPRK SVLPARTELR RGDWKTDSMS STASSTSNRS STRSLLSVSS GMEGDNEDNE IPEITRSRGP GPTQVDGAPV VTGTPVGTLE RPPRVPPRAA SQRPLTRESF HPPPPVPPRG R Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PIK3AP1 (phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1))

Alternative Name: Pik3ap1

Background: Phosphoinositide 3-kinase adapter protein 1 (B-cell adapter for phosphoinositide 3-kinase) (B-cell phosphoinositide 3-kinase adapter protein 1),FUNCTION: Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL. {ECO:0000269|PubMed:11781242, ECO:0000269|PubMed:11877477, ECO:0000269|PubMed:12833156, ECO:0000269|PubMed:18025150, ECO:0000269|PubMed:18337558, ECO:0000269|PubMed:22187458, ECO:0000269|PubMed:22187460}.

Molecular Weight: 90.9 kDa

UniProt: Q9EQ32

AlphaFold: Q9EQ32

Pathways: Activation of Innate immune Response, BCR Signaling, Warburg Effect