UBE3B Protein (AA 1-1070) (His tag)

Catalog No. ABIN7565058

Product Details

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Protein Type: Recombinant

Protein Characteristics: AA 1-1070

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This UBE3B protein is labelled with His tag.

Purpose: Made-to-order recombinant Ube3b Protein expressed in mammalian cells.

Sequence: MFTVSQTSRA WFIDRARQAR EERLVQKERE RSAVTIQALV RSFLCRRRLH RDIRKEIDEF FSADESGSSK RSALCIFKIA RRLLFICKTT EDSERLEKLC RSILNSMDAE NEPKVWYVSL ALSKDLTLLW IKQIKSILWH CCELLGQLKP EILQDSRLIT LYLTMLVTFT DTSTWKILRG KGESLRPALN HICANIMGHL NQRGLYSVLQ VLLTRGLARP RPCLSKGMLT AAFSLALRPV VAAQFSDNLM RPFIIHVMSV PALVAHLSTV APERLGVLES HDMLRKFIVF LRDRDRCRDA CESLEGCHTL CLMGNLLHLG SLSLRLLEEE MDGFVSALTQ MLCYCQKYVA QKKSNLTHWH PVLGWFSQPV DYGLNDSMYL ITKQLQFLWA VPLIRILFSD ILSRKLLEHA EPAPVQPQPS SPQTVLPVKS LLKRAFQKSA SVRNILRPVG GRRVDSAEVR KVCNICVLYQ TSLTTLTQIR LQILTGLTYL DDLLPKLWAF ICELGPHGGL KLFLECLNND TGESKQLLAM LMLFCDCSRH LITILDDIEV YEEQISFKLE ELVTISSFLN SFVFKMIWDG IVENAKGETL ELFQSVHGWL MVLYERDCRR RFAPEDHWLR RDLKPGVLFQ ELDKDRRRAQ LVLQHIPHVV PHKNRVLLFR NMVIKEKEKL GLVETSSASP HVTHITIRRS RMLEDGYEQL RQLSQHAMKG VIRVKFVNDL GVDEAGIDQD GVFKEFLEEI IKRVFDPALN LFKTTSGDER LYPSPTSYIH ENYLQLFEFV GKMLGKAVYE GIVVDVPFAS FFLSQMLGHH HSVFYSSVDE LPSLDSEFYK NLTSIKRYDG DIADLGLTLS YDEDVMGQLV CHELVPGGKT IPVTDENKIS YIHLMAHFRM HTQIKNQTAA LISGFRSIIK PEWIRMFSTP ELQRLISGDN AEIDLEDLKK HTVYYGGFHG SHRVIIWLWD ILASDFTPEE RAMFLKFVTS CSRPPLLGFA YLKPPFSIRC VEVSDDQDTG DTLGSVLRGF FTIRKREPGG RLPTSSTCFN LLKLPNYSKK SVLREKLRYA ISMNTGFELS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Alternative Name: Ube3b (UBE3B Products)

Background: Ubiquitin-protein ligase E3B (EC 2.3.2.26) (HECT-type ubiquitin transferase E3B),FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. {ECO:0000250}.

Molecular Weight: 122.8 kDa

UniProt: Q9ES34

AlphaFold: Q9ES34