CDC20 Protein (AA 1-499) (His tag)

Catalog No. ABIN7565098

This Recombinant CDC20 protein is produced in HEK-293 Cells.

Quick Overview for CDC20 Protein (AA 1-499) (His tag) (ABIN7565098)

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-499

Purification tag / Conjugate: This CDC20 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Cdc20 Protein expressed in mammalian cells.

Sequence: MAQFVFESDL HSLLQLDAPI PNAPVARWQR KAKEATGPAP SPMRAANRSH SAGRTPGRTP GKSSSKVQTT PSKPGGDRFI PQRSASQMEV ASFLLSKENQ PEDRGTPTKK EHQKAWSLNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKTCRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY LAVGTSNAEV QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWAPLQT FTQHQGAVKA VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLGLT MSPDGATVAS AAADETLRLW RCFEMDPALR REREKASVAK SSLIHQGIR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Alternative Name: Cdc20

Background: Cell division cycle protein 20 homolog (mmCdc20) (p55CDC),FUNCTION: Involved in the metaphase/anaphase transition of cell cycle (PubMed:32666501). Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (PubMed:34298015). {ECO:0000269|PubMed:19900895, ECO:0000269|PubMed:32666501, ECO:0000269|PubMed:34298015}.

Molecular Weight: 54.8 kDa

UniProt: Q9JJ66

AlphaFold: Q9JJ66