HEY2 Protein (AA 1-339) (His tag)

Catalog No. ABIN7565132

This Recombinant HEY2 protein is expressed in HEK-293 Cells.

Quick Overview for HEY2 Protein (AA 1-339) (His tag) (ABIN7565132)

Target: HEY2 (Hairy/enhancer-of-Split Related with YRPW Motif 2 (HEY2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-339

Purification tag / Conjugate: This HEY2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Hey2 Protein expressed in mammalian cells.

Sequence: MKRPCEETTS ESDLDETIDV GSENNYPGHA TSSVMRSNSP TTTSQIMARK KRRGIIEKRR RDRINNSLSE LRRLVPTAFE KQGSAKLEKA EILQMTVDHL KMLQATGGKG YFDAHALATD FMSIGFRECL TEVARYLSSV EGLDPSDPLR VRLVSHLSTC ASQREAAVMT SSMAHHHHPL HPHHWAAAFH HLPTALLQPN GLHTSESTPC RLSTSSEVPS AHGSALLTAT FAHADSALRM PSGGTVAPCV PPLSTSLLSL SATVHAAAAA ATAAAHSFPL SFAGAFPMLP SNAAAAAAVA AATAISPPLS VSAASSPQQT STGTNNKPYQ PWGTEVGAF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: HEY2 (Hairy/enhancer-of-Split Related with YRPW Motif 2 (HEY2))

Alternative Name: Hey2

Background: Hairy/enhancer-of-split related with YRPW motif protein 2 (HES-related repressor protein 2) (Hairy and enhancer of split-related protein 2) (HESR-2) (Hairy-related transcription factor 2) (HRT-2) (mHRT2) (Protein gridlock homolog),FUNCTION: Transcriptional repressor which functions as a downstream effector of Notch signaling in cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY1 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3. {ECO:0000269|PubMed:10692439, ECO:0000269|PubMed:11095750, ECO:0000269|PubMed:11160397, ECO:0000269|PubMed:11486045, ECO:0000269|PubMed:12372253, ECO:0000269|PubMed:12372254, ECO:0000269|PubMed:12454287, ECO:0000269|PubMed:12947105, ECO:0000269|PubMed:15107403, ECO:0000269|PubMed:15297376, ECO:0000269|PubMed:15345511, ECO:0000269|PubMed:15485867, ECO:0000269|PubMed:15680351, ECO:0000269|PubMed:16199874, ECO:0000269|PubMed:17259303, ECO:0000269|PubMed:17303760, ECO:0000269|PubMed:17332425}.

Molecular Weight: 35.9 kDa

UniProt: Q9QUS4

AlphaFold: Q9QUS4

Pathways: Regulation of Muscle Cell Differentiation