CHST4 Protein (AA 1-388) (His tag)

Catalog No. ABIN7565187

Product Details

Target: CHST4 (Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase 4 (CHST4))

Protein Type: Recombinant

Protein Characteristics: AA 1-388

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CHST4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Chst4 Protein expressed in mammalian cells.

Sequence: MMLLKKGRLL MFLGSQVIVV ALFIHMSVHR HLSQREESRR PVHVLVLSSW RSGSSFVGQL FGQHPDVFYL MEPAWHVWMT FTSSTAWKLH MAVRDLLRSV FLCDMSVFDA YMNPGPRKQS SLFQWEQSRA LCSAPVCDFF PAHEISSPKH CKLLCGQQPF DMVEKACRSH GFVVLKEVRF LSLQALYPLL TDPSLNLHVV HLVRDPRAVF RSREHTTIEL VVDSHIVLGQ HLETIKEEDQ PYYAMKIICK SQVDIVKAIQ TLPEALQQRY LFLRYEDLVR APLAQTTRLY KFVGLDFLPH LQTWVHNVTR GKGMGQHAFH TNARNALNVS QAWRWSLPYE KVSQLQDACG EAMDLLGYLQ VRSQQEQGNL SLDLLSSSHI LGQVFREG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CHST4 (Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase 4 (CHST4))

Alternative Name: Chst4 (CHST4 Products)

Background: Carbohydrate sulfotransferase 4 (EC 2.8.2.-) (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3) (GST-3) (High endothelial cells N-acetylglucosamine 6-O-sulfotransferase) (HEC-GlcNAc6ST) (L-selectin ligand sulfotransferase) (LSST) (N-acetylglucosamine 6-O-sulfotransferase 2) (GlcNAc6ST-2) (Gn6st-2),FUNCTION: Sulfotransferase involved in SELL/L-selectin ligand biosynthesis pathway. Catalyzes the transfer of the sulfate group from 3'-phospho-5'-adenylyl sulfate (PAPS) onto the hydroxyl group at C-6 position of the non-reducing N-acetylglucosamine (GlcNAc) residue within O-linked mucin-type glycans (PubMed:10435581, PubMed:16227985, PubMed:15319280, PubMed:16227986, PubMed:14597732, PubMed:15111310). Contributes to generate sialyl 6-sulfo Lewis X determinant (also known as MECA-79 epitope) for SELL recognition, a prerequisite for continuous lymphocyte homing into peripheral lymph nodes and antigen immune surveillance. Transfers the sulfate group primarily on core 2 GlcNAcbeta1-6(Galbeta1-3)GalNAcalphaSer/Thr and extended core 1 GlcNAcbeta1-3Galbeta1-3GalNAcalphaSer/Thr based O-linked glycans on CD34 and GLYCAM1 peripheral node addressins (PNAds) expressed on the lumenal side of high endothelial venules (HEVs) (PubMed:10435581, PubMed:16227985, PubMed:15319280, PubMed:16227986, PubMed:15111310, PubMed:14593101). The recognition of PNAds by SELL initiates a multistep process comprising tethering and rolling of blood lymphocytes on HEVs against the blood flow, followed by chemokine signaling, integrin-mediated lymphocyte adhesion onto endothelial cells and lymphocyte transendothelial migration. Modulates rolling velocity and differential T and B lymphocyte recruitment into peripheral lymph nodes, with a major role in B lymphocyte homing (PubMed:16227985, PubMed:15319280, PubMed:16227986, PubMed:14597732, PubMed:10435581). Might be redundant in sulfation of MADCAM1 and lymphocyte trafficking to mesenteric lymph nodes (PubMed:10435581, PubMed:11520459, PubMed:16227986). Can also sulfonate core 3 GlcNAcbeta1-3GalNAc-R based glycans as well as GlcNAcbeta1-3Galbeta1-Glc, GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharides, which might be ectopically expressed during tumorigenesis (By similarity). {ECO:0000250|UniProtKB:Q8NCG5, ECO:0000269|PubMed:10435581, ECO:0000269|PubMed:11520459, ECO:0000269|PubMed:14593101, ECO:0000269|PubMed:14597732, ECO:0000269|PubMed:15111310, ECO:0000269|PubMed:15319280, ECO:0000269|PubMed:16227985, ECO:0000269|PubMed:16227986}.

Molecular Weight: 44.6 kDa

UniProt: Q9R1I1

AlphaFold: Q9R1I1