MBD1 Protein (AA 1-636) (His tag)

Catalog No. ABIN7565297

Product Details

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-636

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This MBD1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Mbd1 Protein expressed in mammalian cells.

Sequence: MAESWQDCPA LGPGWKRRES FRKSGASFGR SDIYYQSPTG EKIRSKVELT RYLGPACDLT LFDFRQGTLC HPIPKTHPLA VPSKKKKKPS KPAKTKKQQV GLQRSEVRIE TPQGEYKAPT ATALASLSVS ASASSSASAS ASASSHAPVC CENCGIHFSW DGVKRQRLKT LCKDCRAQRI AFNREQRMFK RVGCGDCAAC LVKEDCGVCS TCRLQLPSDV ASGLYCKCER RRCLRIMEKS RGCGVCRGCQ TQEDCGHCCI CLRSPRPGLK RQWRCLQRRC FWGKRDSSKR GSKVASQRHS QAPPLPPHPA SQYTEPTELH ISDIAPTSPA EFIYYCVDED EDELQPYTNQ RQNRKCGACA ACLRRMDCGR CDFCCDKPKF GGGNQKRQKC RWRQCLQFAM KRLLPSAGSG SGEGAGLRPY QTHQTHQKRP ASARQLQLSS PLKAPWAVVT APPGPVRDSR KQQAGRGSVL PQPDTDFVFL QEGTSSAMQM PGTAAASTEV PVQAAQCSAP SWVVALPQVK QETADAPEEW TAVTTFLTSS TLQSGFPSKA ADPDLSPVKQ EPPGPEEDGE EKKDDVSETT PAEEIGGVGT PVITEIFSLG GTRLRDAEAW LPRLHKLLAV NEKEYFTELQ LKEEVL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: Mbd1 (MBD1 Products)

Background: Methyl-CpG-binding domain protein 1 (Methyl-CpG-binding protein MBD1),FUNCTION: Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 can also repress transcription from unmethylated promoters. {ECO:0000269|PubMed:15060159, ECO:0000269|PubMed:9774669}.

Molecular Weight: 70.0 kDa

UniProt: Q9Z2E2

AlphaFold: Q9Z2E2