Elf4 Protein (AA 1-655) (His tag)

Catalog No. ABIN7565301

Recombinant Elf4 protein expressed in HEK-293 Cells.

Quick Overview for Elf4 Protein (AA 1-655) (His tag) (ABIN7565301)

Target: Elf4 (E74 like ETS transcription factor 4 (Elf4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-655

Purification tag / Conjugate: This Elf4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Elf4 Protein expressed in mammalian cells.

Sequence: MAIALQPSDL VFEFASNGMD DIHQLEDPSV FPAVIVEQVP YPELVHLCSG LDLDEVHNGI IRDRTLCMTQ DQILEGSILL TDDDVSTSNN VSSTEVLFNV ATPSDVLDEK QIFSSPEVLS DSNSVQAINL PNFLLSTPEP DDLKKTSDAG DQKEHSEEEK VSREENLRKM GKARKRNRKT KNNRSTSPVT DPSMPIRKKS KDGKGSTIYL WEFLLALLQD RNTCPKYIKW TQREKGIFKL VDSKAVSKLW GKQKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLVVID DEEESPETPE DSSQASTSST PSTSTIRRAS SRVGTRASPE DKDNPPWEKP KVQHTGLQPS ASLELGLSVD EEVPTTSTML ASPLQSQARL TKTVSSSPAP SNIHLGVAPV GPGSTVTLQT IPLTTVLTNG PPASTTAPTQ LVLQSVPQVS TFKDTFTLQT SFPLNTNLQE NQVATQGAPL ILSGLPQLLA GANPQSNPAP SQVIGAGSAG PSSQPPGTVI AAFIRTSSGT SVPVVKEGPL RSSSYVQGVV TGAPVEGLLV PEETLRELLR DQGHLQPLPS QVLSRGSHNL SLVGNQTLSP PSHPTVGLTP VAELELSSGS GPLFVTEPSV TRSPTQAPFS PFNPTSLIKM EPQDI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Elf4 (E74 like ETS transcription factor 4 (Elf4))

Alternative Name: Elf4

Background: ETS-related transcription factor Elf-4 (E74-like factor 4) (Myeloid Elf-1-like factor),FUNCTION: Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of LYZ. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Transactivates the PRF1 promoter in natural killer (NK) cells and in CD8+ T cells (PubMed:34326534). Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2. Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway. Is a transcriptional regulator of inflammation, controlling T-helper 17 (Th17) cells and macrophage inflammatory responses. Required for sustained transcription of anti-inflammatory genes, including IL1RN (PubMed:34326534, PubMed:35266071). Is a negative regulator of pro-inflammatory cytokines expression, including IL17A, IL1B, IL6, TNFA and CXCL1 (PubMed:34326534, PubMed:35266071). Down-regulates expression of TREM1, a cell surface receptor involved in the amplification of inflammatory responses (PubMed:34326534, PubMed:35266071). {ECO:0000250, ECO:0000269|PubMed:12387738, ECO:0000269|PubMed:19380490, ECO:0000269|PubMed:19412182, ECO:0000269|PubMed:34326534, ECO:0000269|PubMed:35266071}.

Molecular Weight: 70.8 kDa

UniProt: Q9Z2U4

AlphaFold: Q9Z2U4

Pathways: Positive Regulation of Endopeptidase Activity