PD-1 Protein (AA 25-167)

Catalog No. ABIN7566100

Recombinant PD-1 protein expressed in HEK-293 Cells.

Quick Overview for PD-1 Protein (AA 25-167) (ABIN7566100)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 25-167

Purpose: CD279 [PD-1] (human) (rec.)

Cross-Reactivity: Human

Characteristics: The extracellular domain of human CD279 [PD-1] (aa 25-167) is untagged.

Sterility: Sterile filtered

Endotoxin Level: <1EU/mg protein (LAL test, Lonza).

Biological Activity Comment: Measured by its binding ability in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: CD279 [PD-1]

Background:

PD-1, Programmed Cell Death Protein 1

CD279 (Programmed Cell Death Protein 1, PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-gamma. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.

Molecular Weight: ~30kDa (SDS-PAGE)

NCBI Accession: NP_005009

Pathways: Cancer Immune Checkpoints