FGF21 Protein (AA 29-209) (DYKDDDDK Tag)

Catalog No. ABIN7566147

Product Details

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Protein Type: Recombinant

Protein Characteristics: AA 29-209

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FGF21 protein is labelled with DYKDDDDK Tag.

Purpose: FGF-21 (human) (rec.)

Cross-Reactivity: Human

Characteristics: Human FGF-21 (aa 29-209) is fused at the C-terminus to a DYKDDDDK Tag.

Purity: >90 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After reconstitution:for 10 μg size: 0.1 mg/mLfor 50 μg size: 1 mg/mL

Concentration: Lot specific

Buffer: Contains PBS.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Alternative Name: FGF-21 (FGF21 Products)

Background:

Fibroblast Growth Factor 21

The fibroblast growth factor (FGF) family is a group of multifunctional signaling molecules that have a wide variety of functions. The family comprises of 22 related proteins, each grouped into subfamilies which are based on genetic and functional similarity. FGF21 is expressed in several tissues such as liver (main site), muscles, adipocytes, pancreas, and brain where it passes the blood-brain barrier. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression. Its activity requires the presence of the FGFR1 and beta-klotho. FGF21 mediates the adaptive starvation response to induce ketogenesis, gluconeogenesis, lipolysis, and lipid beta-oxidation. FGF21 plays also a key role in cardiac remodeling with the heart expressing fibroblast growth factor receptor 1 (FGFR1), beta-klotho, as well as FGF21. In muscle, FGF21 expression in skeletal muscles is due to mitochondrial dysfunction and ER stress.

Molecular Weight: ~22kDa (SDS-PAGE)

UniProt: Q9NSA1

Pathways: RTK Signaling