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FGF21 Protein (AA 29-209) (mFc-Avi Tag,Biotin)

FGF21 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7274636
  • Target See all FGF21 Proteins
    FGF21 (Fibroblast Growth Factor 21 (FGF21))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 29-209
    Origin
    • 18
    • 9
    • 5
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 21
    • 12
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This FGF21 protein is labelled with mFc-Avi Tag,Biotin.
    Purpose
    Biotinylated Human FGF21 Protein
    Sequence
    His29-Ser209
    Characteristics
    Recombinant Biotinylated Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-Terminus.It contains His29-Ser209.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Biotinylated Human FGF21, mFc Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.26μg/ml determined by ELISA. See testing image for detail.
    Top Product
    Discover our top product FGF21 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    FGF21 (Fibroblast Growth Factor 21 (FGF21))
    Alternative Name
    FGF21 (FGF21 Products)
    Synonyms
    FGF21 Protein, fibroblast growth factor 21 Protein, FGF21 Protein, fgf21 Protein, Fgf21 Protein
    Background
    Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
    Molecular Weight
    46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
    UniProt
    Q9NSA1
    Pathways
    RTK Signaling
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